Running "Manual" Application » History » Revision 3
Revision 2 (Amber Herold, 04/27/2010 10:36 AM) → Revision 3/6 (Anchi Cheng, 05/22/2010 11:44 PM)
h1. Running "Manual" Application * Leginon/Manual> Open the "Settings" tool !http://emg.nysbc.org/software/leginon/images/icons/settings.png! and configure the settings: > > > Important-Use low dose kit option should be set to yes if using the low dose kit on Tecnai. Low Dose Pause Time need to be set to 5 sec or more to complete the mode change. > > > Recommandations-Acquisition/Camera Recommandations- Acquisition/Camera Configuration- use one of the preconfigured selection for binning and size > > > Correct image=yes if dark and bright images of the camera configuration exist. > > > Save image to the database=yes. * Leginon/manual/Settings> Save and close the settings by pressing "OK" button. * Adjust the exposure time in the setting and/or beam size/spot size on the scope to achieve the desired dose and coverage in exposure mode, if low-dose kit is used. * Microscope> Search the grid for interested area and focus as normally performed. * Microscope> If low dose mode is used, leave TEM in Focus model. Leave the screen at up position if "Main Screen/Up before acquire" is not checked in the settings. No need to put on beam blank. * Leginon/manual> Click on the "Acquire" tool !http://emg.nysbc.org/software/leginon/images/icons/acquire.png! to acquire the image. * Microscope> If low dose mode is used, Leginon will force the pre-specimen shutter close, switch the scope to exposure mode to acquire the image and then switch back to the original mode. ______ [[Startup|< Startup]] | [[Advanced Features|Advanced Features >]] ______