Setup df preset » History » Version 2
Anchi Cheng, 04/29/2020 06:17 PM
| 1 | 1 | Anchi Cheng | h1. Setup df preset |
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| 3 | h2. Microscope setup for df preset |
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| 5 | 2 | Anchi Cheng | 1. Center the smallest C2 aperture you have. |
| 6 | 2. Center the beam and set in nanoprobe parallel illumication and eucentric focus in SA range imaging mode. |
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| 7 | 3. Change spot size to larger number such as spot 10 and center this beam is needed through Direct Alignment reset Beam Shift. |
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| 8 | 4. Change gun lens to get much weaker beam. For example from 3.3 to 7.1 reduces the beam current to 25%. You probably won't be able to see the beam any more on the flucam. |
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| 9 | 5. Push the diffraction button on the control panel to put this in diffraction mode. |
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| 10 | 6. Select desired camera length |
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| 11 | 7. Push the eucentric button on the control panel to focus the beam on the diffraction plane. |
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| 12 | 8. Insert beam stop and center the diffraction beam behind it. |
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| 13 | 1 | Anchi Cheng | |
| 14 | h2. Camera setup for df preset |
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| 16 | dimension: 2048 x 2048 |
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| 17 | binning: 2 x 2 |
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| 18 | exposure time: 1000 ms (not used during movie collection, but used for DPreview node) |
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| 20 | h2. Cycle through hl and df presets a few times to allow it to settle on lens normalization before adjusting df preset diffraction shift again. Repeat until satisfied. |