Sub-3Å proteasome image-shift data collection » History » Version 1
Anchi Cheng, 08/16/2016 05:59 PM
| 1 | 1 | Anchi Cheng | h1. Sub-3Å proteasome image-shift data collection |
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| 3 | h2. Instruments |
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| 5 | SEMC@NYSBC Krios |
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| 6 | # Scope: FEI Titan-Krios, 300 kV, micro probe at low mag, nano probe at high mags |
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| 7 | # Camera: Gatan K2. mount below Falcon/Ceta. 1.21 Å at 29kx scope nominal mag |
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| 9 | h2. Grid |
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| 11 | * 1.2/1.3 C-flat carbon support; 400 mesh Copper grid. |
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| 13 | [[Krios1-K2-NYSBC]] |
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| 15 | h2. Application: |
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| 17 | MSI-T2 Advanced (= MSI-T + "Beam Tilt Image" for coma-free alignment + "N2 Filling" for Krios auto nitrogen filler) |
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| 19 | h2. Presets Manager: |
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| 21 | No preset cycling (Krios has its own normalization). |
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| 23 | h2. Queuing usage: |
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| 25 | Hole Targeting: Queuing, target at the carbon area to cover four holes. |
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| 26 | Exposure Targeting: *No Queuing*. automated targeting. See [[Exposure Targeting Set-up for MSi-T for Four Holes]] |
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| 28 | h2. Move method to reach the targets: |
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| 30 | Hole: [[Iterative_Stage_Movement|Navigator Iterative movement]]: Target Tolerance: 1e-7 m; Acceptable Tolerance: 3e-7 m |
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| 31 | Focus: Presets Manager image shift |
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| 32 | Exposure: Presets Manager image shift |
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| 34 | h2. Wait time before final exposure: |
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| 35 | 3 seconds |
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| 36 | 10 seconds addition for the first target |