Summary of MSI applications » History » Revision 6
Revision 5 (Anchi Cheng, 06/18/2010 03:42 PM) → Revision 6/7 (Amber Herold, 06/21/2010 03:54 PM)
h1. Summary of MSI applications All the MSI applications share shares the same scheme. They are 5-stage acquisition processes which include a grid atlas, squares acquisition, holes (called subsquare in MSI-Raster) acquisition, focusing, and final exposures (Tomography for MSI-Tomography). Target finding at each stage leads to acquisition at the next stage. Drift management, eucentric height correction, and autofocusing is also involved. The diagram below shows the flow of control in MSI. The detailed event bindings involved are for advanced users only and can be found in the chapter covering creating/editing application at the end of the full manual. !http://emg.nysbc.org/software/leginon/images/images/MSI_flow.png! The The black arrows show shows the flow of execution from the excutation that the node where the arrow starts on initiation from initiate the execution of execution to the node that the arrow is pointed to. The green arrow shows the control of target shift correction by drift manager. The red,blue, purple, and magenta frames enclose nodes that processes acquisition, focus, reference and preview targets, respectively. These nodes set sets presets through Presets Manager and moved to their targets using either Presets Manager or Navigation node. The green frames are nodes for drift management. managerment. The following list is a cursory glance at the steps to run this application: * Calibrations (autofocus, image shift, stage position, modeled stage position, ...) * Set-up Presets (gr, sq, hl, fa, fc, en, ef) * Acquire Corrector images. * Acquire a grid atlas. * Select squares on the grid atlas. The process can be wholly wholely automated from this point until more squares are selected. * Optionally interact with the hole finders. * Optionally putting targets from different images at the same level into queue that can be processed in a batch. This chapter has been divided into sections to facilitate everyday usage: [[Pre-MSI Set-up|Pre-MSI Set-up]]: Prior to setting up Leginon for the day, make sure that the presets have been designed and Leginon calibrated for your purpose. [[Initial MSI application preference setup|MSI preference & configuration]]: The comprehensive MSI preferences and configuration example. [[MSI Quick-start|MSI Quick Start checklist]]: A short check list for users who are familiar with the application and have saved preferences. [[MSI set-up in more details|MSI set-up in more details]]: The more complicated MSI Leginon application set-up that should be completed before collecting data. [[Queuing option|MSI queuing option]]: Introduction to the queuing option. [[Queuing Example 1 - Exposure Targeting|MSI Exposure Target queuing]]: The setup and operation of queuing mode for large number of exposure targets and/or for avoiding accessing sq presets after initial acquisition and targeting. [[Queuing Example 2 - Hole Targeting only|MSI Hole Target queuing]]: The setup and operation of queuing mode recommanded for quick overall survey of rare hole targets in grid squares. [[Queuing_Example_3_-_Both_Exposure_and_Hole_Targeting|Queuing at both levels]]: The setup and operation of queuing mode recommanded for more advanced user who will wait for one operation done before proceeding. [[Trouble shooting|MSI Trouble shooting]]: This trouble shooting section addresses some of the possible challenges a Leginon user may face while using the MSI application. ______ [[Flavors of MSI applications|< Flavors of MSI applications]] | [[Pre-MSI Set-up|Pre-MSI Set-up >]] ______