Test Leginon with Simulator » History » Version 17
Anchi Cheng, 02/02/2011 12:24 PM
| 1 | 1 | Amber Herold | h1. Test Leginon with Simulator |
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| 5 | h2. Test Leginon with Simulator |
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| 9 | TEM and CCD simulators are created automatically on the computer Leginon program started |
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| 10 | at. They are convenient as a way to look around the GUI to try out buttons. The images |
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| 11 | "acquired" are just random squares on background. Here we will use them to test the database |
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| 12 | connection and storage disk mapping. |
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| 16 | h2. Start Leginon on the computer you will run most of the process |
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| 20 | 15 | Amber Herold | # Register as a Leginon user and then copy and personalize your configuration file before your first use. |
| 21 | 17 | Anchi Cheng | ** Leginon guru: See [[Recommendation for setup at a new institute]] |
| 22 | ** Regular Users: Refer to [[Set up for a new regular user|Set up for a new regular user]] in "Administration Tools" Chapter. |
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| 23 | 15 | Amber Herold | |
| 24 | # start-leginon.py (From any directory for Linux) |
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| 26 | 14 | Amber Herold | OR |
| 27 | 15 | Amber Herold | |
| 28 | 14 | Amber Herold | from Start Menu: \Start\Programs\Leginon> Leginon (on Windows) |
| 29 | 15 | Amber Herold | |
| 30 | # Leginon Setup Wizard> select "Create session" in session type if a new session is needed. |
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| 31 | |
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| 32 | 1 | Amber Herold | ** Fill in session name and comments. Comments will appear in the Web Viewers |
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| 34 | 1 | Amber Herold | ** Select instrument and project, if project database is used. Image path is automatically selected. |
| 35 | 15 | Amber Herold | |
| 36 | 1 | Amber Herold | ** Image path is automatically selected based on your leginon.cfg file. |
| 37 | 15 | Amber Herold | |
| 38 | # Leginon Setup Wizard> No need to edit client list as the computer where the program is started is automated added. |
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| 40 | # Choose "Finish" in the set up wizard to start the session. |
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| 44 | h2. Acquiring the first simulated image |
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| 48 | 15 | Amber Herold | # Leginon/Application/Run> select Manual Application. |
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| 50 | # Leginon> From the list of nodes on the left panel, select Manual node. |
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| 52 | # Leginon/Manual> Open the "Setting" tool and configure the settings: |
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| 54 | 1 | Amber Herold | ** SimuTEM and SimuCCDCamera should be available for selection. |
| 55 | 15 | Amber Herold | |
| 56 | 12 | Amber Herold | ** Acquisition/Camera Configuration- use one of the preconfigured selection for binning and size such 512x8. |
| 57 | 15 | Amber Herold | |
| 58 | 12 | Amber Herold | ** Save image to the database = yes. |
| 59 | 15 | Amber Herold | |
| 60 | 1 | Amber Herold | ** Leave the rest of the configuration as default. |
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| 62 | |
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| 63 | # You should see your first simulated image acquired through Leginon in the image display panel. |
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| 66 | 7 | Alex Kadokura | |
| 67 | h2. Check on the Web viewer |
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| 68 | 1 | Amber Herold | |
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| 70 | 12 | Amber Herold | |
| 71 | 1 | Amber Herold | Follow the [[Using the Web viewer|instruction in Manual Application Chapter]]. |
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| 74 | h2. Problem solving for the simulation test |
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| 76 | 9 | Alex Kadokura | |
| 77 | 10 | Alex Kadokura | Most problems regarding this test are caused by bad configuration. You should double |
| 78 | 11 | Anchi Cheng | check your [[Configure leginon.cfg|leginon.cfg]], [[Configure sinedon.cfg|sinedon.cfg]], [[Web Server Installation|config.php]] as outlined in |
| 79 | 1 | Amber Herold | complete installation chapter. See [[Test run operation problems|Installation |
| 80 | 16 | Amber Herold | Troubleshooting]], and the "Leginon Forum":http://emg.nysbc.org/projects/leginon/boards/6 or "Web Tools Forum":http://emg.nysbc.org/projects/leginon/boards/10 such as the topic " "information not saved to db":http://emg.nysbc.org/boards/10/topics/686" |
| 81 | 1 | Amber Herold | for common problems. |
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| 84 | 2 | Amber Herold | If everything goes smoothly, do a test directly on the computer controlling the microscope as in the next section. |
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| 88 | ______ |
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| 90 | [[Test Leginon on the Computer Controlling the Microscope|Test Leginon on the Computer Controlling the Microscope >]] |
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| 92 | 1 | Amber Herold | ______ |