Test Leginon with Simulator » History » Version 22
Anchi Cheng, 04/04/2018 03:56 PM
| 1 | 1 | Amber Herold | h1. Test Leginon with Simulator |
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| 5 | h2. Test Leginon with Simulator |
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| 9 | TEM and CCD simulators are created automatically on the computer Leginon program started |
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| 10 | at. They are convenient as a way to look around the GUI to try out buttons. The images |
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| 11 | "acquired" are just random squares on background. Here we will use them to test the database |
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| 12 | connection and storage disk mapping. |
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| 14 | 20 | Anchi Cheng | h2. Configure a local instruments.cfg to be used with the simulators |
| 15 | 1 | Amber Herold | |
| 16 | 20 | Anchi Cheng | Copy pyscope/instruments.cfg.template to your home directory and rename it instruments.cfg |
| 17 | 1 | Amber Herold | |
| 18 | 20 | Anchi Cheng | |
| 19 | 19 | Anchi Cheng | h2. Start Leginon on the computer you will run the process |
| 20 | 1 | Amber Herold | |
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| 23 | 15 | Amber Herold | # Register as a Leginon user and then copy and personalize your configuration file before your first use. |
| 24 | 18 | Anchi Cheng | ** Leginon guru: See [[Recommandation_for_setup_at_a_new_institute|Recommendation for setup at a new institute]] |
| 25 | 17 | Anchi Cheng | ** Regular Users: Refer to [[Set up for a new regular user|Set up for a new regular user]] in "Administration Tools" Chapter. |
| 26 | 15 | Amber Herold | |
| 27 | # start-leginon.py (From any directory for Linux) |
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| 28 | |
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| 29 | 14 | Amber Herold | OR |
| 30 | 15 | Amber Herold | |
| 31 | 14 | Amber Herold | from Start Menu: \Start\Programs\Leginon> Leginon (on Windows) |
| 32 | 15 | Amber Herold | |
| 33 | # Leginon Setup Wizard> select "Create session" in session type if a new session is needed. |
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| 34 | |
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| 35 | 1 | Amber Herold | ** Fill in session name and comments. Comments will appear in the Web Viewers |
| 36 | 15 | Amber Herold | |
| 37 | 1 | Amber Herold | ** Select instrument and project, if project database is used. Image path is automatically selected. |
| 38 | 15 | Amber Herold | |
| 39 | 1 | Amber Herold | ** Image path is automatically selected based on your leginon.cfg file. |
| 40 | 15 | Amber Herold | |
| 41 | # Leginon Setup Wizard> No need to edit client list as the computer where the program is started is automated added. |
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| 42 | |
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| 43 | # Choose "Finish" in the set up wizard to start the session. |
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| 44 | 1 | Amber Herold | |
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| 47 | h2. Acquiring the first simulated image |
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| 51 | 15 | Amber Herold | # Leginon/Application/Run> select Manual Application. |
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| 53 | # Leginon> From the list of nodes on the left panel, select Manual node. |
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| 55 | # Leginon/Manual> Open the "Setting" tool and configure the settings: |
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| 57 | 1 | Amber Herold | ** SimuTEM and SimuCCDCamera should be available for selection. |
| 58 | 15 | Amber Herold | |
| 59 | 12 | Amber Herold | ** Acquisition/Camera Configuration- use one of the preconfigured selection for binning and size such 512x8. |
| 60 | 15 | Amber Herold | |
| 61 | 12 | Amber Herold | ** Save image to the database = yes. |
| 62 | 15 | Amber Herold | |
| 63 | 1 | Amber Herold | ** Leave the rest of the configuration as default. |
| 64 | 15 | Amber Herold | |
| 65 | |
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| 66 | # You should see your first simulated image acquired through Leginon in the image display panel. |
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| 67 | 1 | Amber Herold | |
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| 69 | 7 | Alex Kadokura | |
| 70 | h2. Check on the Web viewer |
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| 71 | 1 | Amber Herold | |
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| 73 | 12 | Amber Herold | |
| 74 | 1 | Amber Herold | Follow the [[Using the Web viewer|instruction in Manual Application Chapter]]. |
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| 76 | 22 | Anchi Cheng | It will looks like the image below. The lack of metadata is because no calibration was done. Not a problem. |
| 77 | !simulator_view.png! |
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| 79 | 21 | Anchi Cheng | h2. Undo the local instruments configuration |
| 80 | 1 | Amber Herold | |
| 81 | 21 | Anchi Cheng | When you are satisfied, remove the instruments.cfg from your home directory as it would show up in real microscopy sessions as well. |
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| 85 | 1 | Amber Herold | h2. Problem solving for the simulation test |
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| 87 | 9 | Alex Kadokura | |
| 88 | 1 | Amber Herold | Most problems regarding this test are caused by bad configuration. You should double |
| 89 | 20 | Anchi Cheng | check your [[Configure leginon.cfg|leginon.cfg]], [[Configure sinedon.cfg|sinedon.cfg]], [[Web Server Installation|config.php]] as outlined in |
| 90 | complete installation chapter. See [[Test run operation problems|Installation |
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| 91 | Troubleshooting]], and the "Leginon Forum":http://emg.nysbc.org/projects/leginon/boards/6 or "Web Tools Forum":http://emg.nysbc.org/projects/leginon/boards/10 such as the topic " "information not saved to db":http://emg.nysbc.org/boards/10/topics/686" |
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| 92 | for common problems. |
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| 93 | 1 | Amber Herold | |
| 94 | 12 | Amber Herold | |
| 95 | 2 | Amber Herold | If everything goes smoothly, do a test directly on the computer controlling the microscope as in the next section. |
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| 99 | ______ |
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| 101 | [[Test Leginon on the Computer Controlling the Microscope|Test Leginon on the Computer Controlling the Microscope >]] |
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| 103 | 1 | Amber Herold | ______ |