TiltPicker Changelog¶

v2.0 -- March XX, 2010
NOTES:
TiltPicker bugs are now tracked via the AMI redmine server, visit
http://emg.nysbc.org/projects/tiltpicker/
to file any bugs
BUGS: * Particle picking outside image area is now not allowed * Made sure all functions are available from the top menu * When saving a file, it checks the extension and adds one if it does not exist * Shortened button row, for smaller screens * Fixed polygon tool for rectagular images * Fixed reversal of tilt axis angles * Fixed improper print of DoG picker particle contrast messages on command line. * Faster polygon removal
FEATURES: * Added transfer picks option (Alt-X) * Added complete features for DoG picker:
- specify multiple size
- show DoG maps in GUI for advanced threshold/size tweaking * Added particle cutoff tool to remove all particles with error above cutoff * Users can now open new images from the menu * Added About TiltPicker screen * Print version and citation info upon launch on command line * Users can now reject the results from DoG picker if the picks were bad * A dialog asks for confirmation to reset or clear parameters * Added TiltPick2Spi.py program to split single TiltPicker file into 3 spider files * Changed default shapes to differently sized circles, this is customizable on command line * Added more dialogs and checking to prevent user error * Better documentation in docs/USAGE.txt

v1.0 -- Jan 22, 2009 * Better output file writing; completely rewritten * Can now both read and write SPIDER, Text and Pickle files * Add all button the menus at the top * Set minimum window size to work well on 1024x768 laptop * Fixed problem with DoG picker on rectangular images * Removed numextension requirement from polygon tool * Enable guess shift functionality * Better bad picks processing * Ability to read SPIDER images, did not realize this was missing * Added ApTiltAutoPicker.py, type --help to see options * Set SPIDER file format as the default output * Published paper:
N.R. Voss, C.K. Yoshioka, M. Radermacher, C.S. Potter and B. Carragher
"DoG Picker and TiltPicker: software tools to facilitate particle
selection in single particle electron microscopy."
DOI: http://dx.doi.org/10.1016/j.jsb.2009.01.004

v0.9.1 -- May 22, 2008 * Fixed bug when saving files of uneven number of particles

v0.9 -- May 21, 2008 * Added aligned x,y to txt format * Removed radermacher module requirement, but still recommended * Removed numextension module requirement * Change mask to not alter levels so much * Fixed crashed when only one worst pick is chosen * Fixed guess xy-shift for large tilts * Set max peaks in DoG picker * Range to search for particle pair is limited by particle size in DoG picker * ~_{Sort particles based on distance from upper-left corner; helps for finding unmatched particles}~ * Added "Auto Optimize" button, to auto run "Find Theta", "Optimize Angles", and "Mask Region" * Set upper limit of bad particles to 10 * Fixed overlap import to database

v0.8 -- Feb 1, 2008 * Removed 'from gui.wx', should fix some importing bugs * Reset mask with reset button * Add DoG auto picking (experimental) * Add clear bad picks & remove clear all picks buttons * Show particles with largest error 4*stdev and min filter * Added number of triangles used to find theta dialog * Changed bad picks behavior to always highlight worst particle * Added 'Guess Shift' for pipeline tilt picker * Calculates percent overlap when mask is selected * Added selection polygon removal tool * Better particle pick importing no more orphans * Better polygon removal no more orphans * Tried to organize buttons better, still needs work * Does allow you to quit with uneven # of particles * Bad picks shows unmatched particles

v0.7 -- Dec 5, 2007 * Added FAQ based on emails I have received * Add error values to text file output * Auto-save feature to prevent against crashing * Show particles with largest error: if error > mean+3*stdev then yellow cross * Fix for xy-shifting * Fix for import Leginon tilt data * Fix for error values in text file

v0.6.4 -- Nov 27, 2007 * Removed requirement for apVersion from apParam

v0.6.3 -- Nov 27, 2007 * Removed requirement for sinedon and sinedon.cfg from apParam * Changed gamma and phi angles to agree with WEB * Fixed mask regions for non-square images * Remove save buttons from bottom button row to make room for future buttons

v0.6.2 -- Nov 20, 2007 * Fixed reading of JPEG, PNG, TIFF images * Clean up help function * Created ChangeLog file

v0.6.1 -- Nov 19, 2007 * Fix for module installations * Created INSTALL file * Added Apache 2.0 LICENSE file * Ctrl-A does 'apply' NOT 'save as'

v0.6 -- Nov 7, 2007 * Major feature changes * Use optparse for command line parsing * Fix SPIDER commenting * Allow adjusting of icon shapes & sizes

v0.5 -- Oct 2, 2007 * First full-featured release