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Ctf Display Tool failing while running CTFFIND4 with phas... » appion_ctffind4_output.txt

Rick Baker, 05/03/2017 02:12 PM

 
Starting image 2 ( skip:8, remain:2 ) id:695438, file: 17may02a_e6f_012gr_01sq_014hl_01en-a
... Pixel size: 1.2
Found 0 ctf values
!!! WARNING: no best CTF value for image 17may02a_e6f_012gr_01sq_014hl_01en-a
!!! WARNING: Defocus minimum is less than zero
Defocus search range: 1000 A to 45000 A (0.10 to 4.50 um)
... running ctf estimation at Tue May 2 13:28:47 2017
input = 17may02a_e6f_012gr_01sq_014hl_01en-a.mrc
output = 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc
apix = 1.2
kv = 200.0
cs = 2.7
ampcontrast = 0.1
fieldsize = 1024
resmin = 50.0
resmax = 4.0
defmin = 1000.0
defmax = 45000.0
defstep = 1000.0
known_astig = no
exhaustive_astig_search = no
restrain_astig = yes
expect_astig = 500.0
phase = yes
min_phase_shift = 0.174532925199
max_phase_shift = 2.96705972839
phase_search_step = 0.174532925199
expert_opts = no
newline =


/data/software/repo/ctffind4/4.0.16/ctffind4
17may02a_e6f_012gr_01sq_014hl_01en-a.mrc
17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc
1.2
200.0
2.7
0.1
1024
50.0
4.0
1000.0
45000.0
1000.0
no
no
yes
500.0
yes
0.174532925199
2.96705972839
0.174532925199
no


** Welcome to CTFFind **
Version - 4.0.16
Input Mode: Batch
Date & Time: 2017-05-02 13:28:47


Copyright 2015 Howard Hughes Medical Institute. All rights reserved.
Use is subject to Janelia Farm Research Campus Software Copyright 1.1
license terms ( http://license.janelia.org/license/jfrc_copyright_1_1.html )

Input image file name
[17may02a_e6f_012gr_01sq_013hl_01en-a.mrc] : 17may02a_e6f_012gr_01sq_014hl_01en-a.mrc
Output diagnostic filename
[17may02a_e6f_012gr_01sq_013hl_01en-a-pow.mrc] : 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc
Pixel size [1.2] : 1.2
Acceleration voltage [200.0] : 200.0
Spherical aberration [2.7] : 2.7
Amplitude contrast [0.1] : 0.1
Size of power spectrum to compute [1024] : 1024
Minimum resolution [50.0] : 50.0
Maximum resolution [4.0] : 4.0
Minimum defocus [1000.0] : 1000.0
Maximum defocus [45000.0] : 45000.0
Defocus search step [1000.0] : 1000.0
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : yes
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : 500.0
Find additional phase shift? [yes] : yes
Minimum phase shift [0.174532925199] : 0.174532925199
Maximum phase shift [2.96705972839] : 2.96705972839
Phase shift search step [0.174532925199] : 0.174532925199

Summary information for file 17may02a_e6f_012gr_01sq_014hl_01en-a.mrc
Number of columns, rows, sections: 3710 3838 1
MRC data mode: 2
Bit depth: 32
Pixel size: 1.000 1.000 1.000

Working on micrograph 1 of 1
100% [==============================] done!
Estimated defocus values : 20788.59 , 20508.88 Angstroms
Estimated azimuth of astigmatism: -65.43 degrees
Additional phase shift : 1.121 (.36 pi)
Score : .09671
Thon rings with good fit up to : 3.6 Angstroms

Summary of results : 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.txt
Diagnostic images : 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc
Detailled results, including 1D fit profiles: 17may02a_e6f_012gr_01sq_014hl_01en-a-pow_avrot.txt
Use this command to plot 1D fit profiles : ctffind_plot_results.sh 17may02a_e6f_012gr_01sq_014hl_01en-a-pow_avrot.txt
Total execution time : 1 minute and 56 seconds
2017-05-02 13:30:43 : CTFFind finished cleanly.
... ctf estimation completed in 1 min 56 sec
... reading 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.txt
... reading MRC: 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc size:(1024, 1024) dtype:float32
... writing JPEG: 17may02a_e6f_012gr_01sq_014hl_01en-a-pow.jpg
==== Committing data to database ====
... Committing ctf parameters for 17may02a_e6f_012gr_01sq_014hl_01en-a to database
... Reading image...
[CTF param] def1: 2.051 um | def2: 2.079 um | angle: 24.6 | ampcontr 0.10 | defratio 1.014
[CTF param] additional phase shift: 64.2 degrees
Final params: def1: 2.051 um | def2: 2.079 um | angle: 24.6 | defratio 1.01
... Defocus Astig Percent Diff 0.68 -- 2.051, 2.079 um
Computing power spectra in 2048x2048 blocks........
... Compute PSD with 8 subfields and fieldsize 2048 complete in 5.11 sec
* correlation 1: 0.04181 -> contribution: 0.1303
* correlation 2: 0.03923 -> contribution: 0.1223
* correlation 3: 0.04326 -> contribution: 0.1348
* correlation 4: 0.04218 -> contribution: 0.1314
* correlation 5: 0.04049 -> contribution: 0.1262
* correlation 6: 0.03840 -> contribution: 0.1197
* correlation 7: 0.03809 -> contribution: 0.1187
* correlation 8: 0.03742 -> contribution: 0.1166
Using 8 of 8 subfields in final power spectra
... Preform a rotational average and remove spikes...
PART 1: SETUP PARAMETERS AND ELLIPTICAL AVERAGE
Resolution range for the data is 1/68.5A to 1/2.0A
... far from focus images, increasing number of extrema (100)
... Number of available peaks is 117
... setting the number of sections to default: 6
... Determine and subtract noise model
PART 2: BACKGROUND NOISE SUBTRACTION
... starting peak extension
Peak Extension Complete in 1.87 msec
PART 3: ENVELOPE NORMALIZATION
... Setting last 3 values to -3.2 (test = -3.2)
... starting peak extension
Peak Extension Complete in 1.92 msec
PART 3B: BAND-PASS FILTER
PART 4: PEAK EXTENSION
... starting peak extension
Peak Extension Complete in 1.78 msec
... starting peak extension
Peak Extension Complete in 1.79 msec
PART 5: CTF FIT AND CONFIDENCE
1/30A - 1/10A confidence is 0.989 (overfocus 0.156)
1/30A - 1/10A confidence is 0.989 (overfocus 0.156)
5 peak confidence is 0.979 (overfocus 0.066)
PART 6: CTF RESOLUTION LIMITS
[ 0.898 0.763 0.764 -0.076 0.062 0.091 0.188 0.199 0.245 0.279
0.269 0.262 0.193 0.176 0.151]
!!! WARNING: Res calc: Negative values in first 10 values
[ 0.898 0.763 0.764 -0.076 0.062 0.091 0.188 0.199 0.245 0.279
0.269 0.262 0.193 0.176 0.151]
!!! WARNING: Res calc: Negative values in first 10 values
Resolution limit is 4.01 at 0.8 and 3.56 at 0.5
PART 7: MAKE 1D PLOT SUMMARY FIGURE
... Saving 1D graph to file 17may02a_e6f_012gr_01sq_014hl_01en-a-plots.png
PART 8: NORMALIZE THE 2D IMAGE
... converting 1d arrays to 2d and normalizing
.................. ... 2d image normalization complete
... Resolution ring of the edge of the image 2.84
... Resolution ring of 25th valley 6.400
(5.0, 4.0086544986979424, 3.5551356021786837, 6.3996785830426726)
... Final resolution ring of 2D Plot: 4.621
PART 9: DRAW THE 2D POWERSPEC IMAGE
... Saving 2D powerspectra to file: 17may02a_e6f_012gr_01sq_014hl_01en-a-powerspec.jpg
Full CTF display makeCtfImages routine complete in 16.45 sec
17may02a_e6f_012gr_01sq_014hl_01en-a-powerspec.jpg
[CTF run] method: unknown | runname ctffind4run1
[CTF param] def1: 2.051 um | def2: 2.079 um | angle: 24.6 | ampcontr 0.10 | defratio 1.014
[CTF param] additional phase shift: 64.2 degrees
[CTF stats] conf_30-10: 0.989 | conf_5peak: 0.979 | res_0.8: 4.0A | res_0.5 3.6A

SUMMARY: ctffind4
------------------------------------------
TIME: 2 min 13 sec
AVG TIME: 1.5 +/- 0.55 min
(- REMAINING TIME: 2 min 3 sec for 1 images -)
------------------------------------------

Starting image 3 ( skip:8, remain:1 ) id:695440, file: 17may02a_e6f_012gr_01sq_015hl_01en-a
... Pixel size: 1.2
Found 0 ctf values
!!! WARNING: no best CTF value for image 17may02a_e6f_012gr_01sq_015hl_01en-a
!!! WARNING: Defocus minimum is less than zero
Defocus search range: 1000 A to 45000 A (0.10 to 4.50 um)
... running ctf estimation at Tue May 2 13:31:00 2017
input = 17may02a_e6f_012gr_01sq_015hl_01en-a.mrc
output = 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc
apix = 1.2
kv = 200.0
cs = 2.7
ampcontrast = 0.1
fieldsize = 1024
resmin = 50.0
resmax = 4.0
defmin = 1000.0
defmax = 45000.0
defstep = 1000.0
known_astig = no
exhaustive_astig_search = no
restrain_astig = yes
expect_astig = 500.0
phase = yes
min_phase_shift = 0.174532925199
max_phase_shift = 2.96705972839
phase_search_step = 0.174532925199
expert_opts = no
newline =


/data/software/repo/ctffind4/4.0.16/ctffind4
17may02a_e6f_012gr_01sq_015hl_01en-a.mrc
17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc
1.2
200.0
2.7
0.1
1024
50.0
4.0
1000.0
45000.0
1000.0
no
no
yes
500.0
yes
0.174532925199
2.96705972839
0.174532925199
no


** Welcome to CTFFind **
Version - 4.0.16
Input Mode: Batch
Date & Time: 2017-05-02 13:31:00


Copyright 2015 Howard Hughes Medical Institute. All rights reserved.
Use is subject to Janelia Farm Research Campus Software Copyright 1.1
license terms ( http://license.janelia.org/license/jfrc_copyright_1_1.html )

Input image file name
[17may02a_e6f_012gr_01sq_014hl_01en-a.mrc] : 17may02a_e6f_012gr_01sq_015hl_01en-a.mrc
Output diagnostic filename
[17may02a_e6f_012gr_01sq_014hl_01en-a-pow.mrc] : 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc
Pixel size [1.2] : 1.2
Acceleration voltage [200.0] : 200.0
Spherical aberration [2.7] : 2.7
Amplitude contrast [0.1] : 0.1
Size of power spectrum to compute [1024] : 1024
Minimum resolution [50.0] : 50.0
Maximum resolution [4.0] : 4.0
Minimum defocus [1000.0] : 1000.0
Maximum defocus [45000.0] : 45000.0
Defocus search step [1000.0] : 1000.0
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : yes
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : 500.0
Find additional phase shift? [yes] : yes
Minimum phase shift [0.174532925199] : 0.174532925199
Maximum phase shift [2.96705972839] : 2.96705972839
Phase shift search step [0.174532925199] : 0.174532925199

Summary information for file 17may02a_e6f_012gr_01sq_015hl_01en-a.mrc
Number of columns, rows, sections: 3710 3838 1
MRC data mode: 2
Bit depth: 32
Pixel size: 1.000 1.000 1.000

Working on micrograph 1 of 1
100% [==============================] done!
Estimated defocus values : 22200.57 , 21926.01 Angstroms
Estimated azimuth of astigmatism: -54.36 degrees
Additional phase shift : 1.489 (.47 pi)
Score : .09369
Thon rings with good fit up to : 3.9 Angstroms

Summary of results : 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.txt
Diagnostic images : 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc
Detailled results, including 1D fit profiles: 17may02a_e6f_012gr_01sq_015hl_01en-a-pow_avrot.txt
Use this command to plot 1D fit profiles : ctffind_plot_results.sh 17may02a_e6f_012gr_01sq_015hl_01en-a-pow_avrot.txt
Total execution time : 1 minute and 57 seconds
2017-05-02 13:32:57 : CTFFind finished cleanly.
... ctf estimation completed in 1 min 58 sec
... reading 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.txt
... reading MRC: 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc size:(1024, 1024) dtype:float32
... writing JPEG: 17may02a_e6f_012gr_01sq_015hl_01en-a-pow.jpg
==== Committing data to database ====
... Committing ctf parameters for 17may02a_e6f_012gr_01sq_015hl_01en-a to database
... Reading image...
[CTF param] def1: 2.193 um | def2: 2.220 um | angle: 35.6 | ampcontr 0.10 | defratio 1.013
[CTF param] additional phase shift: 85.3 degrees
Final params: def1: 2.193 um | def2: 2.220 um | angle: 35.6 | defratio 1.01
... Defocus Astig Percent Diff 0.62 -- 2.193, 2.220 um
Computing power spectra in 2048x2048 blocks........
... Compute PSD with 8 subfields and fieldsize 2048 complete in 5.0 sec
* correlation 1: 0.03175 -> contribution: 0.1312
* correlation 2: 0.03356 -> contribution: 0.1386
* correlation 3: 0.02944 -> contribution: 0.1216
* correlation 4: 0.02893 -> contribution: 0.1195
* correlation 5: 0.02790 -> contribution: 0.1153
* correlation 6: 0.02753 -> contribution: 0.1137
* correlation 7: 0.03229 -> contribution: 0.1334
* correlation 8: 0.03070 -> contribution: 0.1268
Using 8 of 8 subfields in final power spectra
... Preform a rotational average and remove spikes...
PART 1: SETUP PARAMETERS AND ELLIPTICAL AVERAGE
ERROR
!!! WARNING: Logging images failing CtfdisplayTool to /data/microscopes/appion/17may02a/ctf/ctffind4run1/failed_ctfdisplay_images.txt
[CTF run] method: unknown | runname ctffind4run1
[CTF param] def1: 2.193 um | def2: 2.220 um | angle: 35.6 | ampcontr 0.10 | defratio 1.013
[CTF param] additional phase shift: 85.3 degrees

SUMMARY: ctffind4
------------------------------------------
TIME: 2 min 7 sec
AVG TIME: 1.55 +/- 0.55 min
------------------------------------------
!!! WARNING: skipped total of 8 images.
... to process them again, remove 'continue' option and run ctffind4 again.
... Finished all images, checking for more

... Querying database for preset 'en-a' images from session '17may02a' ...
... 14 images recevied in 13.65 msec
... Found 14 images in 13.72 msec
... Remove processed images
skipping processed images
............
... finished skipping in 14.07 msec

!!! WARNING: skipped 12 of 14 images
... [[ 0 no reprocess | 0 rejected | 0 wrong tilt | 12 in donedict ]]
... Process images old to new

Beginning Main Loop

Starting image 0 ( skip:12, remain:2 ) id:695442, file: 17may02a_e6f_012gr_01sq_016hl_01en-a
... Pixel size: 1.2
Found 0 ctf values
!!! WARNING: no best CTF value for image 17may02a_e6f_012gr_01sq_016hl_01en-a
!!! WARNING: Defocus minimum is less than zero
Defocus search range: 1000 A to 45000 A (0.10 to 4.50 um)
... running ctf estimation at Tue May 2 13:33:06 2017
input = 17may02a_e6f_012gr_01sq_016hl_01en-a.mrc
output = 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.mrc
apix = 1.2
kv = 200.0
cs = 2.7
ampcontrast = 0.1
fieldsize = 1024
resmin = 50.0
resmax = 4.0
defmin = 1000.0
defmax = 45000.0
defstep = 1000.0
known_astig = no
exhaustive_astig_search = no
restrain_astig = yes
expect_astig = 500.0
phase = yes
min_phase_shift = 0.174532925199
max_phase_shift = 2.96705972839
phase_search_step = 0.174532925199
expert_opts = no
newline =


/data/software/repo/ctffind4/4.0.16/ctffind4
17may02a_e6f_012gr_01sq_016hl_01en-a.mrc
17may02a_e6f_012gr_01sq_016hl_01en-a-pow.mrc
1.2
200.0
2.7
0.1
1024
50.0
4.0
1000.0
45000.0
1000.0
no
no
yes
500.0
yes
0.174532925199
2.96705972839
0.174532925199
no


** Welcome to CTFFind **
Version - 4.0.16
Input Mode: Batch
Date & Time: 2017-05-02 13:33:06


Copyright 2015 Howard Hughes Medical Institute. All rights reserved.
Use is subject to Janelia Farm Research Campus Software Copyright 1.1
license terms ( http://license.janelia.org/license/jfrc_copyright_1_1.html )

Input image file name
[17may02a_e6f_012gr_01sq_015hl_01en-a.mrc] : 17may02a_e6f_012gr_01sq_016hl_01en-a.mrc
Output diagnostic filename
[17may02a_e6f_012gr_01sq_015hl_01en-a-pow.mrc] : 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.mrc
Pixel size [1.2] : 1.2
Acceleration voltage [200.0] : 200.0
Spherical aberration [2.7] : 2.7
Amplitude contrast [0.1] : 0.1
Size of power spectrum to compute [1024] : 1024
Minimum resolution [50.0] : 50.0
Maximum resolution [4.0] : 4.0
Minimum defocus [1000.0] : 1000.0
Maximum defocus [45000.0] : 45000.0
Defocus search step [1000.0] : 1000.0
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : no
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : yes
Please enter a valid number!
Expected (tolerated) astigmatism [500.0] : 500.0
Find additional phase shift? [yes] : yes
Minimum phase shift [0.174532925199] : 0.174532925199
Maximum phase shift [2.96705972839] : 2.96705972839
Phase shift search step [0.174532925199] : 0.174532925199

Summary information for file 17may02a_e6f_012gr_01sq_016hl_01en-a.mrc
Number of columns, rows, sections: 3710 3838 1
MRC data mode: 2
Bit depth: 32
Pixel size: 1.000 1.000 1.000

Working on micrograph 1 of 1
100% [==============================] done!
Estimated defocus values : 23427.16 , 23135.30 Angstroms
Estimated azimuth of astigmatism: -81.92 degrees
Additional phase shift : 1.643 (.52 pi)
Score : .08659
Thon rings with good fit up to : 17.1 Angstroms

Summary of results : 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.txt
Diagnostic images : 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.mrc
Detailled results, including 1D fit profiles: 17may02a_e6f_012gr_01sq_016hl_01en-a-pow_avrot.txt
Use this command to plot 1D fit profiles : ctffind_plot_results.sh 17may02a_e6f_012gr_01sq_016hl_01en-a-pow_avrot.txt
Total execution time : 1 minute and 56 seconds
2017-05-02 13:35:04 : CTFFind finished cleanly.
... ctf estimation completed in 1 min 58 sec
... reading 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.txt
... reading MRC: 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.mrc size:(1024, 1024) dtype:float32
... writing JPEG: 17may02a_e6f_012gr_01sq_016hl_01en-a-pow.jpg
==== Committing data to database ====
... Committing ctf parameters for 17may02a_e6f_012gr_01sq_016hl_01en-a to database
... Reading image...
[CTF param] def1: 2.314 um | def2: 2.343 um | angle: 8.1 | ampcontr 0.10 | defratio 1.013
[CTF param] additional phase shift: 94.1 degrees
Final params: def1: 2.314 um | def2: 2.343 um | angle: 8.1 | defratio 1.01
... Defocus Astig Percent Diff 0.63 -- 2.314, 2.343 um
Computing power spectra in 2048x2048 blocks........
... Compute PSD with 8 subfields and fieldsize 2048 complete in 5.11 sec
* correlation 1: 0.02584 -> contribution: 0.1422
* correlation 2: 0.02471 -> contribution: 0.1359
* correlation 3: 0.02521 -> contribution: 0.1387
* correlation 4: 0.01857 -> contribution: 0.1022
* correlation 5: 0.02517 -> contribution: 0.1385
* correlation 6: 0.02021 -> contribution: 0.1112
* correlation 7: 0.02322 -> contribution: 0.1278
* correlation 8: 0.01885 -> contribution: 0.1037
Using 8 of 8 subfields in final power spectra
... Preform a rotational average and remove spikes...
PART 1: SETUP PARAMETERS AND ELLIPTICAL AVERAGE
ERROR
!!! WARNING: Logging images failing CtfdisplayTool to /data/microscopes/appion/17may02a/ctf/ctffind4run1/failed_ctfdisplay_images.txt
[CTF run] method: unknown | runname ctffind4run1
[CTF param] def1: 2.314 um | def2: 2.343 um | angle: 8.1 | ampcontr 0.10 | defratio 1.013
[CTF param] additional phase shift: 94.1 degrees

SUMMARY: ctffind4
------------------------------------------
TIME: 2 min 7 sec
AVG TIME: 1.59 +/- 0.55 min
(- REMAINING TIME: 2 min 8 sec for 1 images -)
------------------------------------------

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