What about tilting?
Added by Anonymous about 19 years ago
OK, my third and final post of the day!
Anyone think it possible to use Leginon to collect tilt-pair data?
I can think of a few problems: the worst being image movement when you go to high tilts (even when the eucentric height is adjusted as best you can). Also focussing may be a problem unless you are bang on the tilt axis.
How's that for a challenge!
Replies (1)
- Added by Jim Pulokas about 19 years ago
Leginon 1.2 will be released in a few days and will have the ability to focus on a tilted specimen. We have designed it so that you can choose the focus target and the acquisition targets anywhere, not just on the tilt axis. It will automatically figure out how to do the proper defocus correction.
We are currently working on a scheme to acquire tilt pairs that would do all targets at one tilt, and then do them all again at the second tilt. We are trying to avoid tilting at every target to avoid drift. Hopefully we can have this available in a few months.
A more traditional tomography node is almost ready for release. It will incorporate the UCSF tomography methods into Leginon. This will allow the usual Leginon target selection and then do a tilt series at each selected target, while correcting the shift associated with tilting.
Jim