Z-Focus Problem, Error message:Can't Set Current CameraState
Added by Anonymous about 20 years ago
Greetings List,
I have been running into an error message when I am trying to operate the Z-focus node with Ice samples.
The error I get is "Can't set current camera state" or something very similar.
Has anyone seen this error message before?
This has led to some strange focusing issues that I would like to resolve.
Thanks,
Jonny Sexton
Kornberg Laboratory
Structural Biology, Stanford University
Replies (1)
- Added by Jim Pulokas about 20 years ago
I think I found the problem and there are two solutions depending on your intent:
a) Set melt time to zero in the z focus node. This is what we do, because we do z focusing at a lower mag like 5000 (hole preset) where melting the ice would probably melt out the whole area we are going to image.
b) If you really need to melt ice in z focus for some reason, let me know because we will need to give you an updated application with a SetCameraEvent event binding from the z focus to the instrument. You can also figure out how to do this on your own by exporting the application to xml, making the addition, and importing it back to the database. If you are brave, try to edit it in the leginon application editor, accessable from Leginon's Application menu. You can also add an event binding to the currently running application (change will not be saved to the DB) by going into the event menu and add the event manually. To do this, select Z Focus on the left, Instrument on the right, and double click the SetCameraEvent to add the binding.