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Focusing multiple times at different locations within exposure targeting

Added by Michael Cianfrocco over 8 years ago

Hello,

This may be a question that already has a solution, but I can't figure out how to execute this.

I'd like to focus on two different sides of a hole, and then collect an exposure within the hole.

Does leginon provide you with this ability to do multiple locations? I know how to do multiple focusing attempts at the same location.

I'm asking because I'm trying to think about developing a focusing scheme for accurate defocus measurements to use the Volta phase plate, using the focusing method shown in Danev & Baumeister, eLIFE 2016, Figure 2A and 2B: http://elifesciences.org/content/5/e13046v1.

Thanks!
Mike


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