Leginon

Hi all,

FEI's direct alignment does not allow coma-free direct alignment with a beam-image shift applied, it will be reset any such shift upon entering this alignment. The same goes for beam shift and rotation center, although those ones have "tomo-alternatives" called tomo beam shift and tomo rotation center, meaning any beam-image shift is nor reset upon entering the alignment. For coma-free alignment this tomo option does not exist.

SerialEM has coma-free alignment and here also this is not allowed with a beam-image shift applied.

I understand that Leginon can also do coma-free alignment: Does Leginon require zero beam-image shift or can Leginon do this with a beam-image shift (e.g. tilt-axis shift for tomography or random conical tilt) applied?

Thanks,

Wim Hagen
EMBL Heidelberg