Errors when staring leginon
Added by Anonymous over 17 years ago
We are having issues with starting the leginon software. There is partial communication between the scope and Leginon. We get a confirmation that the Gatan CCD camera has been inserted but other parameter values like magnification etc are not available. Here is a list of some errors:
Cannot get value of 'film user code'
Cannot get value of ' film exposure type'
Cannot get value of ' beam tilt'
Cannot get value of 'raw image shift'
Cannot get value of ' film exposure time'
Cannot get value of ' spot size'
Cannot get value of ' stage positiion'
Cannot get value of ' main screen position'
Cannot get value of ' film text'
Cannot get value of ' film exposure number'
Cannot get value of ' hihg tension'
Cannot get value of ' stigmator'
Cannot get value of ' film data type'
Cannot get value of ' magnification'
Cannot get value of ' magnification index'
Cannot get value of ' defocus'
Cannot get value of ' small screen position'
Cannot get value of ' column valves'
Cannot get value of ' intensity'
etc......
Thanks,
Poojitha (member of Fred's Lab)
Replies (1)
- Added by Jim Pulokas over 17 years ago
It would take some more debugging to find the problem, but based on the error messages, it looks like you are using a fairly old version of Leginon. (Something before 1.1, I think). My recommendation is to upgrade both Leginon and pyScope to the latest versions from our website:
http://emg.nysbc.org/software/
It is possible that the current version of Tecnai software is not compatible with that older version of Leginon/pyScope. Could you also let me know what version of the Tecnai software you are using.
If you do not have any useful data in your Leginon database, then it is easiest to upgrade the Leginon software and then start with a new empty database. Otherwise there are a few things that need to be done to convert an old Leginon database to a new one.
The latest version of Leginon/pyScope may be all you need to fix this problem, or at least it will make it easier to debug any problems.