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Activating the parameters in Instrument Node

Added by Anonymous almost 18 years ago

I have managed to acquire an uncorrected image using Leginon. However I think there is only partial communication between leginon and the scope. This is because in the instrument node, when I hit the refresh button only the status of the Shutter, External Shutter and Hold type is activated. Other parameters like magnification, intensity etc show no values. What can I do about this??

Thanks,

Poojitha


Replies (1)

- Added by Jim Pulokas almost 18 years ago

I'm not sure what is causing this, but we can try a few things.

First try a direct connection to the Tecnai through pyScope like this:

- Make sure to quit Leginon if you currently have it running.

- Start a Python command line (from windows start menu programs)

- enter the following commands one by one:

from pyScope.tecnai import Tecnai
t = Tecnai()
t.getIntensity()

That should return the current value of Intensity (some value between 0 and 1).

Let me know if any of those lines given an error.

If that succeeds, close the python window and start Leginon again with the Manual application. Now I want to see if another node can communicate with the Instrument node (even though Instrument node seems to not work) Create a Presets Manager node like this:

- Start Leginon, Manual application, make sure Instrument is still not working.

- in the Node menu, select "Create..."

- Select the type of the node to be "PresetsManager", enter in any name you want, like "p" if you want to keep it simple, and click "Create"

- The new node "p" should show up in the node list on the left. Click it so you can see its interface.

- Click the little button that looks like a microscope with a green arrow on it ("Create a new preset from the current instrument state")

- In the pop-up window, make sure your TEM and Camera are selected, enter any name you want, then click "Create"

- your new preset should show up in the Presets list. The values of the current TEM parameters should also show up as part of that preset.

Let me know if any of that causes any errors. Some errors could show up on the Leginon interface, or they may be reported on the black python window in the background, so look there also.

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