Issue with preset alignment
Added by Rick Baker over 6 years ago
Hi,
we have had a problem pop up recently with preset alignment in the Leginon Presets node.
When we try to align fa-hl mag, no image seems to be take for the fa preset (see image). This isn't a problem when I set hl as the reference preset (so hl-fa) or if I continue and go to the hl-sq or sq-gr alignment.
We recently had FEI out to service the microscope and they updated the microscope software. I wonder if this is related?
I'm at a loss how to fix this, as Leginon seems to be controlling the camera fine in all other nodes. We are using Leginon 3.3, btw.
Any thoughts?
Thanks!
Rick
Replies (2)
RE: Issue with preset alignment - Added by Anchi Cheng over 6 years ago
I have seen this before when two camera exchange takes longer than the pause between image acquisition. In this case, it is possible that the microscope work has changed the normalization settings which makes it takes extra time to change preset. You can try to increase the pause time between preset changes to avoid this problem.