Leginon

Sorry I forgot how big the mrc file was. Here is a jpeg of the same image.

Try two things:

1. You can view the most recent bright/dark/norm images of a particular channel in Correction Node, available at all applications. Choose the tem and camera configuration in its settings and pick the type of image and channel at main panel toolbar and then click the tool labeled as "Display Normalized Image". This shows you the most recent reference collected. If they look normal, take a corrected image in the same node should give you a good corrected image.

2. If someone else has taking bright/dark images after your session, you will not be able to see the ones used in your session the way above. In this case, copy the script I attached to the computer where you run Leginon, and run it by starting it in python like this:

python findReferenceImages.py

It will use the installed Leginon and its supporting package to query database and give you the file path of the specific bright/dark/norm images. You can then examine them with your favorite mrc image viewer.

If the gain reference images look fine. Something was wrong during your session that transmitted bad raw image to the computer. I don't have a good explanation for that, especially if it happened to all your images that day. Was there any problem with images that used a different camera configuration?

I increased the exposure time after increasing the spot size and then repeated the corrections. That seemed to do the trick. I believe I was very close to the shutter limit when the exposure time was 20 ms.