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Bug #4037

open

Large target shift between targets when image shift is aligned through preset alignment tool in presets manager

Added by Anchi Cheng over 8 years ago. Updated over 8 years ago.

Status:
New
Priority:
Normal
Assignee:
Category:
-
Target version:
-
Start date:
03/15/2016
Due date:
% Done:

0%

Estimated time:
Affected Version:
Appion/Leginon 3.2
Show in known bugs:
No
Workaround:

Use Navigation node to align presets works.


Description

Specific case:

K2 Summit/Orius: DM version: 2.31.734.0

sq: 927x959 bin 4 560x
hl: 927*959 bin 4 4700x
fc: 927*959 bin 2
ed: 3710x3838 bin 1 39000x

the auto-picked coordinates for taking images do not correspond to the acutal position on the grid. I think I told you recently about this problem .. on a quantifoil 2.2 hole the picked and the actual image are about 1 row off.
Now, when I look at the sq and the progress of the sq (see screens attached), then one can see that the image of the sq and the sq-v01 are acutally translated to each other. would you expect that this might be the cause of the observation (one row off).

All the alignments (matrix) have been redone, etc … so that should be ok.
Also, all the presets were image-shift-aligned to each other - again all fine.
What is interesting tho is that in the final image shift alignment going from sq to gr, the sq is now also exactly one row off when compared to the step before (hl to sq).  All other presets (ed>hl, hl>sq) always stay on the same spot.
Actions #1

Updated by Anchi Cheng over 8 years ago

More clues from Thomas Marlovits

when the presets between hl>sq>gr are aligned, they look just perfect
however, when i go to the navigator and take images at the respective presets then the sq is off as described previously.
Actions #2

Updated by Anchi Cheng over 8 years ago

  • Description updated (diff)
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