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Bug #4898

open

Alternate tilt scheme produces larger error than sequential and tracking failed between 30-40 degree on Krios but not Arctica

Added by Anchi Cheng over 7 years ago. Updated over 7 years ago.

Status:
Assigned
Priority:
Normal
Assignee:
Anchi Cheng
Category:
-
Target version:
Appion - Appion/Leginon 3.3
Start date:
04/11/2017
Due date:
% Done:

0%

Estimated time:
Affected Version:
Appion/Leginon 3.3
Show in known bugs:
No
Workaround:

Description

Reported by Gabe Lander

Hi Anchi,
We're collecting some tomography on the Krios right now & noticing that the alternating tilt scheme yields much worse results than the sequential.
The first attached image contains the plots from a tomogram collected using the sequential scheme, the 2nd is using the alternating scheme. As you can see, the shifts in the y axis are much more dramatic using the alternating scheme, and usually by 30-40 degrees the imaging has wandered off the hole in one of the directions.

We didn't seem to have this problem on the Arctica when we used the alternating scheme, so I'm wondering if this is simply a goniometer issue, and that our Krios goniometer can't handle the back-and-forth tilt movements? Or are we missing something here that we can fix?
Best,
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