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Feature #559

closed

Retry with higher defocus when cross-correlation not possible

Added by Matthias Brunner over 14 years ago. Updated about 9 years ago.

Status:
Closed
Priority:
Normal
Assignee:
-
Category:
-
Start date:
05/25/2010
Due date:
% Done:

0%

Estimated time:
Deliverable:

Description

DriftMonitor reports absurdly high drifts (1-10um/s) when the image is coincidentally being recorded exactly at focus and hence with low contrast. This means that it will cause an adjust target, go into drift monitor again, again reporting absurdly high drifts, and go into an endless loop target adjust / drift monitor. Drift value should be checked for sanity and measurement retried by incrementing the defocus value.

Same with autofocus
If focus is off by +2um, and fa is set to -2um contrast is sometimes very low and correlation is not possible. If this is detected fa should be temporarily set to -3um, -4um, ... and focussing retried.

Actions #1

Updated by Neil Voss over 14 years ago

  • Target version set to Appion/Leginon Future Version
Actions #2

Updated by Matthias Brunner about 14 years ago

This is especially true for cryo images at 300kV...would be great if you could look into this.

Actions #3

Updated by Anchi Cheng about 9 years ago

This effect is achieved by duplicating the same focus sequence step.

Actions #4

Updated by Anchi Cheng about 9 years ago

  • Status changed from New to Closed
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