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Align tilt series » History » Revision 14

Revision 13 (Anke Mulder, 06/03/2010 06:05 PM) → Revision 14/29 (Anke Mulder, 06/03/2010 06:07 PM)

h1. Align tilt series 

 !http://emg.nysbc.org/attachments/267/Picture_62.png! 

 h2. General Workflow: 

 # Select the tiltseries for which a tomogram is to be calculated. 
 # Check the runname and enter a description. 
 # Use the radio button to select Protomo Refinement or IMOD shift-only alignment. 
 # To submit the job to the cluster click the "Align Tilt Series" button.    Alternatively, click on "Just Show Command" to obtain a command that can be pasted into a UNIX shell. 
 !http://emg.nysbc.org/attachments/263/Picture_55.png! 
 # If your job has been submitted to the cluster, a page will appear with a link "Check status of job", which allows tracking of the job via its log-file. This link is also accessible from the "1 running" option under the "Aliogn tilt series" submenu in the appion sidebar. 
 # Now click on the "1 Complete" link under the "Align tilt series" submenu. This opens a summary of all tilt series alignment runs that have been completed for this dataset. 
 !http://emg.nysbc.org/attachments/266/Picture_61.png! 
 # Clicking on the "alignrun id" opens a summary page for the alignment cycles that were run. 
 # Clicking on the "refine cycle" number opens a report page for that cycle including input parameters and directory paths.    Clicking on "Alignment Movie" opens a new web-browser with a movie of the aligned tilt series. 
 # The same alignment cycle can be repeated or another initiated. 
 !http://emg.nysbc.org/attachments/271/Picture_68.png! !http://emg.nysbc.org/attachments/270/Picture_67.png! 

 h2. Notes, Comments, and Suggestions: 


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 [[Tomography|< Tomography]] | [[Create full tomogram|Create Full Tomogram >]] 

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