Project

General

Profile

Appion Home » History » Version 12

Amber Herold, 03/31/2010 03:22 PM

1 1 Eric Hou
2 11 Amber Herold
h1. Complete Installation
3 6 Amber Herold
4
5 1 Eric Hou
6 11 Amber Herold
h2. Four Parts of Leginon System
7 1 Eric Hou
8 6 Amber Herold
9 11 Amber Herold
<graphic fileref="http://emg.nysbc.org/software/leginon/images/images/Leg_Proj_parts.png"
10
format="PNG"/>
11 1 Eric Hou
12 11 Amber Herold
Leginon as a system that we distribute can be divided into three parts:
13 6 Amber Herold
14
15 11 Amber Herold
h2. Processing Server (Python-side)
16 1 Eric Hou
17
18 11 Amber Herold
Python (and some c) scripts that handle instrument control, data acquisition, and
19
processing.
20 1 Eric Hou
21
22
23
24 11 Amber Herold
h2. Database Server (MySQL-side)
25 1 Eric Hou
26 11 Amber Herold
27
a MySQL server that handles the database
28
29
30
31
32
h2. Web Server (PHP-side)
33
34
35
This includes php and Java scripts at a webserver that we will create to retrieve image
36
and metadata from the database and file-storage system.
37
38
39
40
41
h2. File Server
42
43
44
This is up to you to set up to store lots of data coming out of Leginon system.
45
46
47
48
49
50
51
h2. What is in this chapter
52
53
54
55
Leginon runs under both the Linux and Microsoft Windows Operating systems. However, the
56
current php-mrctool can not be installed on Windows which means mrc images can not be viewed
57
on the web. See the section on Possible Computer Set-up Configurations for details.
58
59
60
The many components for the Leginon system are divided into packages and need to be
61
installed separately. It has been integrated with several third party software packages that
62
are necessary for its operation. The installation documentation will describe how to set-up
63
Leginon and give hints as to how to install and set-up the necessary third-party software.
64
This documentation is not intended to support the additional, but necessary third-party
65
software.
66
67
68
There has been interest in installing Leginon under Mac OS X. While this is possible in
69
theory, we have not been successful in installing a fully functional system. The two main
70
problems are (1) compilation of php-mrctool from the pre-installed php and (2) wxPython on Mac
71
is unable to hide bitmap objects which makes Leginon graphical user interface difficult to
72
use.
73
74
75 12 Amber Herold
See <link linkend="InstT_install">Installation Troubleshooting</link> and "Leginon Bulletin Board":http://emg.nysbc.org/bb/viewforum.php?f=2searching
76 11 Amber Herold
for "install" if you run into problems.
77
78
79
80
81
h2.  Possible Computer Set-up Configurations
82
83
84
85
Computer(s) used for the whole Leginon system need to support five functions:
86
*  TEM/Camera control and data output (by python-side packages of Leginon
87
system)
88
89
90
*The computer attached to the microscope/camera is used for this function.
91
Therefore, it is on a Windows computer.
92
93
94
*  General Leginon operation such as target selection and image processing (processing
95
server of Leginon system)
96
97
98
*  Image data storage.
99
100
101
*  Other meta-data storage through MySQL database (by database server of Leginon
102
system)
103
104
105
*  Web server (using PHP-side of Leginon system).
106
107
108
*Important* The MRC module that is used to load mrc formatted files to the web
109
pages is not compiled on Windows. Therefore, this function must be on a linux box unless
110
you don't need to see the images***
111
112
113
114
115
116
The five functions can be distributed to five different computers on a network or all on
117
one single computer. However, since the latter minimal setup requires the use of the computer
118
attached to the microscope for all functions, it is not advisable.
119
120
121
Processing server of Leginion is a multi-platform software, meaning it can run on both
122
Microsoft Windows and Linux. It is also inherently designed to utilize distributed systems on
123
a network. MySQL used in the database server is also supported by multiple platforms. This is
124
not true for the Web server because the custom MRC module used in the web viewer only compiles
125
on linux machines. Therefore, one of the computer not part of the microscope must be a linux.
126
The following examples show several arrangements that take advantage of distributed system.
127
Other combinations are possible, but will basically be variations on one of the following
128
themes.
129
130
131
h2.  A: 1 Windows computer (attached to the Microscope) &amp; 1 Linux computer (for all
132
other functions, separate from the Microscope) (Good for single user and small-scale
133
acquisition)
134
135
136
137
138
139
*  Install the latest processing server side of Leginon release (and supporting
140
packages) on the Windows computer controlling the microscope and on the Linux computer
141
which is to run Leginon image acquisition and processing.
142
143
144
*  Install the additional modules needed for CCD camera and film interaction on the
145
Windows computer controlling the microscope.
146
147
148
*  Install MySQL, PHP, the Apache Web Server, and dbem tools only on the Linux
149
computer that will perform these functions, (i.e. NOT the computer controlling the
150
microscope).
151
152
153
154
155
156
157
158
159
h2.  B: 1 Windows computer (attached to the Microscope) &amp; 4 Linux computers (one for
160
each function) (Good for multiple microsopes, users and large-scale acquisition)
161
162
163
164
This is the set-up at NRAMM. It has the processing server side of Leginon installed on
165
the Windows computer attached to the microscope and on the Linux computer that is used for
166
running Leginon. A second Linux machine is dedicated to the database while the web-based
167
viewer is hosted by another server.
168
169
170
171
172
*  Install the python-side Leginon (and supporting packages) on the Windows computer
173
controlling the microscope and on the Linux computer that is to run Leginon. You can
174
make the latter installation accessable to any numbers of linux computers on your
175
network.
176
177
178
*  Install MySQL, PHP, the Apache Web Server, and dbem tools on the second Linux
179
computer that will serve as the database/web server/PHP host.
180
181
182
*  The computer hosting the data storage should be accessable by all linux
183
boxes.
184
185
186
187
188
189
190
191
192
h2.  C: 1 Windows computer (attached to the Microscope), 1 Windows computer running python-
193
and MySQL-side functions and 1 Linux computer as web server (Has been applied
194
successfully)
195
196
197
198
The data storage may be on one of these computers that is accessable by other
199
computers.
200
201
202
203
204
205
206
h2.  Ports that Leginon system uses:
207
208
209
210
This information is useful if you want to open specific ports between computers with a
211
firewall in between.
212
213
214
215
216
* MySQL normally uses port 3306 (but configurable in /etc/my.cnf)
217
218
219
* Leginon Client running on TEM host: port 55555
220
221
222
* Leginon main program on the processing server: dynamically assigned ports between
223
49152 and 65535
224
225
226
There is no strict port assignment since we could potentially have more than one
227
Leginon process running on the same linux host talking to different TEM hosts. It is
228
probably good enough if you only worry about opening up the first few of those ports in
229
your firewall (maybe 49152 through 49160, or something like that).
230
231
232
See more discussion at Leginon bulletin board thread on ""network problem, Leginon not seeing
233
tecnai host":http://emg.nysbc.org/bb/viewtopic.php?t=7"
234
235
236
237
238
239
240
241
242
h2. Where to Register and download Leginon system
243
244
245
246 12 Amber Herold
"http://www.leginon.org/":http://www.leginon.orgis the home. Please
247 11 Amber Herold
register if you want to recieve support from Leginon team.
248
249
250
*  python-side Leginon packages
251
252
253
direct link: "http://emg.nysbc.org/software/leginon/":http://emg.nysbc.org/software/leginon/
254
*  mrctools: including the tools for reading mrc images on the web
255
256
257
direct link: "http://emg.nysbc.org/software/mrctools/":http://emg.nysbc.org/software/mrctools/
258
*  dbemtools: including the tools for administration and webviewing
259
260
261
direct link: "http://emg.nysbc.org/software/dbemtools/":http://emg.nysbc.org/software/dbemtools/
262
*  project tools (optional): For administration and webviewing of projects
263
264
265
direct link: "http://emg.nysbc.org/software/dbemtools/":http://emg.nysbc.org/software/dbemtools/
266
267
268
269
270
h2.  Processing server-side Leginon Installation
271
272
273 1 Eric Hou
274 11 Amber Herold
For the lack of better name, processing server-side Leginon refers to packages that
275
performs the general functions of image acquisition and processing. The instructions here are
276
for Linux. Refer to Windows Installation for Windows specific instructions.
277
278
279
h2. Download svn checkout script from Leginon website
280
281
282
283 12 Amber Herold
"http://www.leginon.org/":http://www.leginon.org/Download
284 11 Amber Herold
svnget-1.6.sh to a convenient location such as your home directory.
285
286
287
288
289
h2. Check out SVN Source Files from the depository
290
291
292
293
run svnget-1.6.sh. It does the
294
following:<programlisting>mkdir Leginon-1.6-ALL
295
cd Leginon-1.6-ALL
296
svn co http://emg.nysbc.org/svn/leginon/branches/1.6 Leginon
297
svn co http://emg.nysbc.org/svn/pyami/branches/leg1.6 pyami
298
svn co http://emg.nysbc.org/svn/pyScope/branches/leg1.6 pyScope
299
svn co http://emg.nysbc.org/svn/sinedon/branches/leg1.6 sinedon
300
svn co http://emg.nysbc.org/svn/numextension/branches/leg1.6 numextension
301
svn co http://emg.nysbc.org/svn/libcv/branches/leg1.6 libcv
302
svn co http://emg.nysbc.org/svn/ImageViewer/branches/leg1.6 ImageViewer</pre>
303
304
305
If the process fails, you probably do not have svn client installed. Skip to "Install
306
the supporting packages first if missing:", then try this again.
307
308
309
310
311
h2.  Perform system check:
312
313
314
315
In addition to the downloads from our svn depository, there are several other
316
requirements that you will get either from your OS installation source, or from its
317
respective website. The system check in the Leginon package checks your system to see if you
318
already have these requirements
319
320
321
322
<pre>cd your_download_area/Leginon-1.6-ALL/Leginon
323
python syscheck.py</pre>
324
325
326
327
If python is not installed, this, of course will not run. If you see any lines like "***
328
Failed...", then you have something missing. Otherwise, everything should result in
329
"OK".
330
331
332
333
334
h2.  Install the supporting packages first if missing:
335
336
337
338
Follow the instruction for your specific Linux distribution.
339
340
341
For example, SUSE users can use YaST to install them; RedHat and CentOS users can use
342
yum.
343
344
345
*SciPy may not build properly on some versions of SuSE due to an incompatible LAPACK
346
package that comes with SuSE. You can get scipy as well as a compatible LAPACK etc. from
347
http://repos.opensuse.org/science (need to specify your SuSE version and machine
348
etc.)
349
350 1 Eric Hou
351 11 Amber Herold
352
353
h2.  Required supporting packages:
354
355
356
|_.Name:|_.Download site:|_.yum package name|_.SuSE rpm name|
357
|Python 2.4 or newer|"http://www.python.org":http://www.python.org|python|python-devel|
358
|wxPython 2.5.2.8 or newer|"http://www.wxpython.org":http://www.wxpython.org|wxPython|python-wxGTK|
359
|MySQL Python client 1.2 or newer|"http://sourceforge.net/projects/mysql-python":http://sourceforge.net/projects/mysql-python|MySQL-python|python-mysql|
360
|Python Imaging Library (PIL) 1.1.4 or newer|"http://www.pythonware.com/products/pil/":http://www.pythonware.com/products/pil/|python-imaging|python-imaging|
361 12 Amber Herold
|Python XML module 0.8.3 or newer|"http://pyxml.sourceforge.net":http://pyxml.sourceforge.net|PyXML|python-xml|
362 11 Amber Herold
|NumPy 1.0.1 or newer|"http://www.scipy.org":http://www.scipy.org|numpy|numpy|
363
|SciPy 0.5.1 (tested, others may work)*|"http://www.scipy.org":http://www.scipy.org, "http://repos.opensuse.org/science":http://repos.opensuse.org/science*|scipy|python-scipy|
364
365
366
367
368
*SciPy may not build properly on some versions of SuSE due to an incompatible LAPACK
369
package that comes with SuSE. You can get scipy as well as a compatible LAPACK etc. from
370
http://repos.opensuse.org/science (need to specify your SuSE version and machine
371
etc.)
372
373
374
375
376
h2.  Optional supporting packages:
377
378
379
380
381
382
*  For using Matlab to explore holefinding algorithm (Matlab HoleFinder)
383
384
385
pymat: "http://sourceforge.net/projects/pymat":http://sourceforge.net/projects/pymat
386
387
388
389
390
391
392
h2. Install the packages you downloaded from svn depository
393
394
395
396
Here are the packages you need to install with python installer inside the
397
Leginon-1.6-ALL folder.
398
399
|_.Name:|_.Purpose:|
400
| Leginon|modular TEM image acquisition|
401
| pyami|general functions|
402
| pyScope|microscope control and monitoring|
403
| sinedon|Leginon/database interaction|
404
| numExtension|c extension for numerical processing|
405
| libCV|small c library of algorithm from computer vision field|
406
| ImageViewer|image viewing for tomography|
407
408
409
410
411
412
413
*  run syscheck.py again to make sure you have everything.
414
415
416
*  Install the package in <emphasis role="bold">each folder</emphasis> with commands
417
like
418
these<pre>cd /your_download_area/Leginon-1.6-ALL/Leginon
419
python setup.py install
420
421
cd ../pyami
422
python setup.py install
423
cd ../pyScope
424
python setup.py install
425
cd ../sinedon
426
python setup.py install
427
cd ../numextension
428
python setup.py install
429
cd ../libcv
430
python setup.py install
431
cd ../ImageViewer
432
python setup.py install</pre>
433
434
435
436
437
438
439
440
441
h2.  python-site-package-path: where the installed python
442
packages went:
443
444
445
Python installer put the packages you installed to its site-packages directory. This
446
enable all users on the same computer to access them. The easiest way to find where your
447
installed package is called by python is to load a module from the package using interactive
448
python command lines like this:
449
450
451
452
453
*  Start python command line from shell
454
455
456
457
<pre>> python</pre>
458
459
460
461
*  Import a module from the package. Let's try sinedon here. All packages installed
462
through the above setup.py script should go to the same place.
463
464
465
466
<pre>python> import sinedon</pre>
467
468
469
470
*  If the module is loaded successfully, call the module attribute __path__ (two
471
underscrolls before "path" and two underscrolls after) will return the location of the
472
module it is loaded from
473
474
475
476
<pre>python> sinedon.__path__
477
478
RETURNS, For example,
479
python> &lt;module 'sinedon' from '/usr/lib/python2.5/site-packages/sinedon/__init__.pyc'> </pre>
480
481
482
In this case, /usr/lib/python2.5/site-packages/ is your python-site-package-path.
483
If you go to that directory, you will find all the packages you just installed.
484
485
486
487
488
489
490
491
492
h2.  Configure leginon.cfg:
493
494
495
Leginon can be configured at three levels:
496
497
498
499
500
*  A skeleton (default) configuration file is available:
501
502
503
504
<pre> _<link linkend="package_path">[python-site-package-path]</link>_/Leginon/config/default.cfg</pre>
505
506
507
508
*  Configurations for all users
509
510
511
512
<pre> _<link linkend="package_path">[python-site-package-path]</link>_/Leginon/config/leginon.cfg</pre>
513
514
515
516
Example of the configuration that you should set: (Here we assume your leginon
517
database is named "dbemdata", and Leginon log into the database as a user named
518
"usr_object" with no password
519
set).<pre>[Images]
520
path: _your_storage_disk_path_/leginon
521
</pre>
522
523
524
*  Configurations for individual users that overrides the all-user configuration if
525
exists
526
527
528
529
<pre> ~/leginon.cfg</pre>
530
531
532
533
This configuration should include all the minimal configuration above and
534
additional configuration for individual user for
535
convenience<pre>[User]
536
Fullname: _your_name_  #The name you entered in the leginon administration web page. Note that it is case sensitive</pre>
537
538
539
540
541
542
543
544
545
h2.  Names used in the example installation
546
547
548
From this point on, you will need to enter database user names for different
549
configuration files and settings. These are NOT Leginon user names but what is required by
550
MySQL database interaction. Consistent names are essential. In the example shown here, the
551
leginon database is called "dbemdata", the project database is called "projectdata". The
552
MySQL user name are the same, "usr_object", and in this case without a password.
553
554
555
556
557
h2.  Configure sinedon.cfg:
558
559
560
Sinedon is designed to be able to interact with multiple databases. It can be
561
configured at global or user level.
562
563
564
565
566
*  An example configuration file is available at:
567
568
569
570
<pre>/your_download_area/Leginon-1.6-ALL/sinedon/examples/sinedon.cfg</pre>
571
572
573
574
*  Configurations for all users should be placed at the following path
575
576
577
578
<pre>  _<link linkend="package_path">[python-site-package-path]</link>_/sinedon/sinedon.cfg</pre>
579
580
581
582
*  Modify host,db,user,passwd to what is used. Note that the user here is MySQL user
583
Leginon uses to communicate with the database for all Leginon users. For Leginon that
584
uses Project database, set the following:
585
586
587
588
<pre>[global]
589
host: _your_database_host_
590
user: usr_object
591
passwd:
592
593
[projectdata]
594
db: projectdata
595
596
[leginondata]
597
db: dbemdata
598
</pre>
599
600
601
602
*  Add database configuration if you intend to use grid-inserting robot. The Robot2
603
module uses the database to communicate to the robot. Applications that carries the
604
name "Robot" requires this to be set. In general, using the same database as the
605
general leginon database is fine.
606
607
608
609
<pre>[robot2]
610
db: dbemdata</pre>
611
612
613
614
615
616
617
618
619
620
621
622
h2. Database Server-side Installation
623
624
625
626
The following is for the computer that hosts the databases. This involves installing MySQL
627
server and creation/configuration of the leginon and project databases.
628
629
630
631
|_.Name:|_.Download site:|
632
|MySQL-Server 5.0 or higher|"http://www.mysql.com":http://www.mysql.com|
633
|MySQL-Client 5.0 or higher|"http://www.mysql.com":http://www.mysql.com|
634
635
636
637
638
h2.  MySQL
639
640
641
642
643
644
*  Install MySQL-Server
645
646
647
648
649
*  Use your package installer (yum, zypper, YaST) if available.
650
651
652
OR
653
654
655
*  Download the latest MySQL-server RPM for Linux from "www.mysql.com":http://www.mysql.com
656
*  Install the MySQL-server rpm:
657
658
659
<pre> rpm -Uvh MySQL-server-5.0.xx-y.i386.rpm</pre>
660
(substitute correct version numbers)
661
662
663
664
665
666
667
*  Install MySQL-Client
668
669
670
671
672
*  Use your package installer (yum, zypper, YaST) if available.
673
674
675
OR
676
677
678
*  Download the latest MySQL-client RPM for Linux from "www.mysql.com":http://www.mysql.com
679
*  Install the MySQL-client rpm:
680
681
682
<pre> rpm -Uvh MySQL-client-5.0.xx-y.i386.rpm</pre>
683
(substitute correct version numbers)
684
685
686
687
688
689
690
*  MySQL configuration file is usually located in /usr/share/mysql. There are
691
several examples there:
692
693
694
695
<pre>&gt; ls /usr/share/mysql/my*
696
/usr/share/mysql/my-huge.cnf
697
/usr/share/mysql/my-innodb-heavy-4G.cnf
698
/usr/share/mysql/my-large.cnf
699
/usr/share/mysql/my-medium.cnf
700
/usr/share/mysql/my-small.cnf
701
&gt; </pre>
702
703
704
705
*  Configure my.cnf in /etc using my-huge.cnf as the template
706
707
708
709
710
* <pre>> cp /usr/share/mysql/my-huge.cnf /etc/my.cnf</pre>
711
712
713
714
*  Edit /etc/my.cnf to add or change query cache variables like these:
715
716
<pre>query_cache_type= 1
717
query_cache_size = 100M
718
query_cache_limit= 100M</pre>
719
720
721
722
723
724
725
726
*  start MySQL Server
727
728
<pre> &gt;/etc/init.d/mysqld start</pre>
729
730
on some installation,
731
732
<pre> &gt;/etc/init.d/mysql start</pre>
733
734
For future reference: start | stop | restart MySQL Server with similar
735
commands:
736
737
<pre> &gt;/etc/init.d/mysqld start
738
&gt;/etc/init.d/mysqld stop
739
&gt;/etc/init.d/mysqld restart</pre>
740
741
If you want to start MySQL automatically at boot on SuSE
742
743
<pre> SuSE &gt;chkconfig mysql on
744
</pre>
745
746
*  For future reference, the database location will be:
747
748
749
750
<pre>&gt; cd /var/lib/mysql
751
Directory: /var/lib/mysql
752
&gt; ls
753
yourdbserver.pid
754
ib_logfile0
755
mysql
756
mysql.sock
757
test
758
&gt; </pre>
759
760
761
762
*  Create leginon database, here we call <link linkend="db_example_names"
763
>dbemdata</link>
764
765
<pre> &gt;mysqladmin create dbemdata</pre>
766
767
768
769
*  Create project database, here we call <link linkend="db_example_names"
770
>projectdata</link> (optional)
771
772
<pre> &gt;mysqladmin create projectdata</pre>
773
774
775
776
*  Connect to mysql db
777
778
<pre>&gt;mysql mysql
779
780
mysql&gt; select user, password, host from user;
781
+------+----------+-----------+
782
| user | password | host      |
783
+------+----------+-----------+
784
| root |          | localhost |
785
| root |          | host1     |
786
|      |          | host1     |
787
|      |          | localhost |
788
+------+----------+-----------+
789
4 rows in set (0.00 sec)</pre>
790
791
792
793
*  Create and grant privileges to a user called <link linkend="db_example_names"
794
>usr_object</link> for the databases on both the localhost and other hosts involved.
795
For example, use wild card '%' for all hosts. You may also set specific privilege to
796
the user. See MySQL Reference Manual for details
797
798
<pre>mysql&gt; create user usr_object@'localhost';
799
mysql&gt; grant all privileges on dbemdata.* to usr_object@'localhost';
800
mysql&gt; grant all privileges on projectdata.* to usr_object@'localhost';</pre>
801
802
Similarly,
803
804
<pre>mysql&gt; create usr_object@'%';
805
mysql&gt; grant all privileges on dbemdata.* to usr_object@'%';
806
mysql&gt; grant all privileges on projectdata.* to usr_object@'%';
807
</pre>
808
809
*  Change Root passworld
810
811
<pre>mysql&gt; update user set password=password('_a passwd_') where user="root";
812
Query OK, 2 rows affected (0.01 sec)
813
Rows matched: 2  Changed: 2  Warnings: 0
814
815
mysql&gt;^D or exit;</pre>
816
From now on, you will need to specify the password to connect to the database as
817
root user like this:
818
819
<pre>>mysql -u root -p mysql</pre>
820
821
<emphasis role="bold">***If your password change does not take effect in your next
822
database connection, you will need to flush the mysql pivileges cache as mysql root
823
and connecting with you old password (or no password in the above
824
case):</emphasis>
825
826
<pre>>mysql -u root mysql</pre>
827
<pre>mysql&gt; flush privileges;
828
mysql&gt;^D or exit;</pre>
829
830
*  Check MySQL variables
831
832
<pre>&gt;mysql -u usr_object dbemdata
833
834
mysql&gt; SHOW VARIABLES LIKE 'query%';
835
+------------------------------+-----------+
836
| Variable_name                | Value     |
837
+------------------------------+-----------+
838
| ft_query_expansion_limit     | 20        |
839
| have_query_cache             | YES       |
840
| long_query_time              | 10        |
841
| query_alloc_block_size       | 8192      |
842
| query_cache_limit            | 104857600 | &lt;&lt;---This should correspond to your change
843
| query_cache_min_res_unit     | 4096      |
844
| query_cache_size             | 104857600 | &lt;&lt;---This should correspond to your change
845
| query_cache_type             | ON        | &lt;&lt;---This should correspond to your change
846
| query_cache_wlock_invalidate | OFF       |
847
| query_prealloc_size          | 8192      |
848
+------------------------------+-----------+
849
10 rows in set (0.00 sec)
850
851
mysql&gt; exit;</pre>
852
853
854
855
*  Make sure MySQL is running
856
857
<pre>prompt:~&gt; mysqlshow
858
+--------------+
859
| Databases    |
860
+--------------+
861
| mysql        |
862
| dbemdata     |
863
| projectdata  |
864
+--------------+</pre>
865
866
867
868
*  Or check with the following php script (if already installed)
869
870
<pre>&lt;?
871
mysql_connect('your_host.your_institute.edu', 'usr_object', '','dbemdata');
872
echo mysql_stat();
873
?&gt; </pre>
874
875
876
Output
877
878
<pre> Uptime: 1452562 Threads: 1 Questions: 618 Slow queries: 0 Opens: 117 Flush tables: 1 Open tables: 106 Queries per second avg: 0.000</pre>
879
880
881
882
883
884
885
886
887
888
h2.  Web server set up and Installation
889
890
891
892
The following applies to the computer that will host the web-accessable image viewers ( a
893
part of dbem tools) and project manager (a part of project tools).
894
895
896
h2. Differences between Linux flavors
897
898
899
900
Different Linux flavors often put web server and mysql-related files in different
901
locations. This can be confusing. From experience, we found the equivalent on CentOS vs
902
SuSE. Here we list them for reference. If your system use a different naming and you are
903
willing to share your experience, please send us the list. We will add it here:
904
905
|_.File or Command Head|_.CentOS|_.SuSE|
906
| php.ini|/etc/| /etc/php5/apache2/|
907
| httpd.conf|/etc/httpd/conf/|/etc/php5/apache2/|
908
| default document_root|/var/www/html/|/srv/www/htdocs/|
909
| apache start/stop/restart command head|/etc/init.d/httpd|/etc/init.d/apache2|
910
| mysql start/stop/restart command head|/etc/init.d/mysqld|/etc/init.d/mysql|
911
912 1 Eric Hou
913
914 11 Amber Herold
915
916
917
h2. Prerequisites for dbemtools, projecttools, and mrctools extension
918
919
920
921
The dbemtools are mostly php scripts that run at the web server. The followings are
922
required before installation of dbemtools and the mrc extension that handles mrc files to be
923
displayed. Some of these packages may be found on the SuSE Linux DVD or included in common
924 1 Eric Hou
package repository. MySQL and the Apache Web Server can be downloaded from their respective
925 11 Amber Herold
websites.
926
927
|_.Name:|_.Download site:|_.yum package name|_.SuSE rpm name|
928
| Apache|"www.apache.org":http://www.apache.org| httpd| apache2|
929
| php|"www.php.net":http://www.php.net| php| php|
930
| php-devel*|"rpmfind.net/linux/RPM/Development_Languages_PHP.html":http://rpmfind.net/linux/RPM/Development_Languages_PHP.html| php-devel| php-devel|
931 12 Amber Herold
| php-gd (including GD library, its development libraries and header *)|"www.php.ned/gd":http://www.libgd.org(Use gd2)| php-gd, gd-devel| php-gd,gd-devel|
932
| fftw3 library (including development libraries and header *)|"www.fftw.org":http://www.fftw.org(Use fftw3.x)| fftw3-devel| fftw3-devel|
933 11 Amber Herold
934
935
936
937
* mrctools are compiled and added to php extension with php-devel package. Mrctools use
938
GD and FFTW3 that need to be compiled from their development libraries while the extension
939
is compiled. If GD and FFTW3 sources were downloaded and compiled directly on your computer,
940
these development files are included. If (as in most cases) GD and FFTW3 are installed from
941
rpm, they are not included. An error message will appear when you attempt to compile
942
mrctools. In this case, you will need separate download and installation of GD-devel and
943 12 Amber Herold
FFTW3-devel. Search "http://rpmfind.net/linux/rpm2html/":http://rpmfind.ned/linux/rpm2html/for GD-devel and FFTW3-devel for the rpm
944 1 Eric Hou
distribution needed for your system.
945 11 Amber Herold
946
947
948
949
h2. Installation tools
950
951
952
953
Use the installation tools available for your linux distribution.
954
955
956
957
958
*  For example, Use yum utility to install.
959
960
961
*  For example, Use the SuSE Linux YaST2 utility or zypper (openSuSE 10.2 and above)
962
to install.
963
964
965
* "A list of required CENTOS
966 12 Amber Herold
rpms and instruction":http://emg.nysbc.org/bb/viewtopic.php?t=238can be found at dbemtools bulletin board at
967 11 Amber Herold
leginon.org.
968
969
970
971
972
973
974
975
976
h2.  PHP, PHP-devel, gd, and fftw3
977
978
979
Install the packages using your installation tools if available. For example, to
980
install gd as php extension you may use
981
982
983
984
<programlisting>CentOS> yum install php-gd
985
SuSE10.2 and above> zypper install php-gd</pre>
986
987
988
989
990
991
h2.  Configure php.ini
992
993
994
995
Edit the following two sections in php.ini (found as /etc/php.ini on CentOS and
996
/etc/php5/apache2/php.ini on SuSE) so that they look like the following:
997
998
999
1000
<pre>register_argc_argv = On</pre>
1001
1002
1003
1004
1005
<pre>short_open_tag = On</pre>
1006
1007
1008
1009
1010
<pre>max_execution_time = 300     ; Maximum execution time of each script, in seconds
1011
max_input_time = 300     ; Maximum amount of time each script may spend parsing request data
1012
memory_limit = 256M      ; Maximum amount of memory a script may consume (8MB)</pre>
1013
1014
1015
1016
You may want to increase max_input_time and memory_limit if the server is heavily used.
1017
At NRAMM, max_input_time=600 and memory_limit=4000M.
1018
1019
1020
1021
1022
h2.  Apache Web Server
1023
1024
1025
1026
1027
1028
*  Install the Apache Web Server with the YaST or yum utility.
1029
1030
1031
*  Find "httpd.conf".
1032
1033
1034
This is /etc/httpd/conf/httpd.conf on CentOS and /etc/php5/apache2/httpd.conf on
1035
SuSE
1036
1037
1038
*  Edit the "httpd.conf" configuration file to look like the following:
1039
1040
1041
1042
<pre>&lt;IfModule mod_dir.c&gt;
1043
DirectoryIndex index.htm index.shtm index.html index.shtml index.php
1044
&lt;/IfModule&gt;</pre>
1045
1046
1047
(Note: It may be possible to edit httpd.conf in YaST2 as well.)
1048
1049
1050
*  Restart the web server.
1051
1052
1053
1054
<pre>apachectl restart
1055
or
1056
/etc/init.d/httpd restart     (ON CentOS)
1057
or
1058
/etc/init.d/apache2 restart   (ON SuSE)</pre>
1059
1060
1061
1062
If you want to start the web server automatically at boot on SuSE
1063
1064
<pre> SuSE &gt;chkconfig apache2 on
1065
</pre>
1066
1067
1068
1069
1070
1071
1072
1073
h2. Check php information
1074
1075
1076
Create the following info.php in your web server document root directory (/var/www/html
1077
on CentOS. /srv/www/htdocs on SuSE. You can find its location in httpd.conf mentioned above
1078
under the line starting
1079
DocumentRoot).<pre>&lt;?php
1080
phpinfo();
1081
?&gt;</pre>
1082
1083
1084
Visit this page at http://yourhost/info.php
1085
1086
1087
You will see comprehensive tables of php and apache information, including the location
1088
of the addition .ini files, extension, include path, and what extension is enabled.
1089
1090
1091
Here is an example screen shot of the part of the info.php page that tells you where
1092
php.ini and other configuration files are.
1093
1094
1095
1096
<graphic fileref="http://emg.nysbc.org/software/leginon/images/images/phpini.png"
1097
format="PNG"/>
1098
1099
1100
1101
1102
1103
h2.  mrctools Installation
1104
1105
1106
1107
mrctools are installed as php extension and are required for displaying mrc files live
1108
on the web browser.
1109
1110
1111
h2.  Install php-devel packages on the web server if missing:
1112
1113
1114
You can check whether php-devel is installed by
1115
typing<pre> &gt;phpize</pre>
1116
1117
1118
Follow the instruction for your specific Linux distribution.
1119
1120
1121
For example, SUSE users can use YaST or zypper to install them
1122
1123
1124
1125
1126
h2.  php-GD/FFTW3-devel
1127
1128 1 Eric Hou
1129 11 Amber Herold
1130
Follow instructions from the download site. These may be included already. If not,
1131
mrctools installation will fail.
1132
1133
1134
1135
1136
h2.  mrctools Installation
1137
1138
1139
1140
mrctools are installed from php devel directory. This is usually /usr/include/php/ext
1141
where you will find other php extension source such as gd. After the installation, mrc.so
1142
should be placed in php's extension directory (Look for "extension_dir" in
1143
http://your_host/<link linkend="install_infophp">info.php</link>
1144
1145
1146
1147
1148
*  Download dbem tools from "http://emg.nysbc.org/software/mrctools":http://emg.nysbc.org/software/mrctools.
1149
1150
1151 12 Amber Herold
*  Follow the instruction at "http://emg.nysbc.org/software/mrctools/mrc_so.php":http://emg.nysbc.org/software/mrctools/mrc_so.phpfor installation and
1152 11 Amber Herold
testing. The next session about checking php information can also help debugging the
1153
installation
1154
1155
1156
1157
1158
1159
1160
1161
1162
h2.  Check php information
1163
1164
1165
1166
Visit or refresh http://yourhost/info.php <link linkend="install_infophp">which you
1167
created earlier</link>. It should have a section looking like this (The version should
1168
correspond to what you've just installed):
1169
1170
1171
1172
<graphic fileref="http://emg.nysbc.org/software/leginon/images/images/phpmrc.png"
1173
format="PNG"/>
1174
1175
1176
1177
If mrc is not listed, the extension did not get added at the right order.
1178
1179
1180
1181
1182
h2.  Alternative approach if mrc module does not show up in info.php output
1183
1184
1185
1186
* find in the <link linkend="install_infophp">info.php web page</link> the location
1187
of "additional .ini files parsed" in the first table (such as
1188
/etc/php.d/conf.d/*).
1189
1190
1191
*  Go to the directory and make a copy of any ini file to use as a template for
1192
mrc.ini
1193
1194
1195
1196
<pre>&gt;cd [additional_ini_directory]
1197
&gt;cp gd.ini mrc.ini</pre>
1198
1199
1200
1201
*  Edit mrc.ini to the following
1202
1203
1204
1205
<pre>; comment out next line to disable mrc extension in php
1206
extension=mrc.so</pre>
1207
1208
1209
1210
*  Comment out mrc extension from php.ini (found in /etc/php.ini/ on a typical PHP
1211
installation)
1212
1213
1214
1215
<pre>;extension=mrc.so</pre>
1216
1217
1218
1219
*  restart your webserver
1220
1221
1222
1223
<pre>&gt; /etc/init.d/httpd restart</pre>
1224
1225
1226
1227
1228
1229
1230
1231
1232
1233
h2.  Web Viewing Tools (dbemtools) Linux
1234
Installation
1235
1236
1237
1238
php and java scripts for viewing images and Leginon information through the web
1239
server.
1240
1241
1242
1243
1244
*  Download dbem tools from "http://emg.nysbc.org/software/dbemtools":http://emg.nysbc.org/software/dbemtools.
1245
1246
1247
*  Unpack the package in your [webdirectory]
1248
1249
1250
1251
<pre>&gt;cd [webdirectory]			#/var/www/html in this example
1252
[webdirectory&gt;tar zxvf dbem_1_5_1.tgz</pre>
1253
1254
1255
1256
*  Copy config.php.template to config.php and edit the latter by adding these MySQL
1257
parameters:
1258
1259
"config.php" should be located in /var/www/html/dbem_1_5_1/ on CentOS and
1260
/srv/www/htdocs/dbem_1_5_1/ on SuSE.
1261
1262
1263
1264
<pre>&lt;?
1265
// ---define dbem web tools base ---//
1266
define('BASE_URL',"/dbem_1_5_1/";
1267
// --- Leginon Viewer Configuration --- //
1268
1269
// --- Set your leginon MySQL database server parameters
1270
1271
$DB_HOST    = "[your database host]";
1272
$DB_USER    = "<link linkend="db_example_names">usr_object</link>";
1273
$DB_PASS    = "";
1274
$DB        = "<link linkend="db_example_names">dbemdata</link>";
1275
1276
// --- XML test dataset
1277
$XML_DATA = "test/viewerdata.xml";
1278
1279
// --- Project database config
1280
1281
$PROJECT_URL = "/project_1_2";
1282
$PROJECT_DB_HOST = "[your database host]";
1283
$PROJECT_DB_USER = "<link linkend="db_example_names">usr_object</link>";
1284
$PROJECT_DB_PASS = "";
1285
$PROJECT_DB = "<link linkend="db_example_names">projectdata</link>";
1286
</pre>
1287
1288
1289
1290
*  Test the set-up by visiting http://yourhost/dbem_1_5_1
1291
1292
1293
1294
1295
1296
1297
1298
1299
h2.  Project management tools Linux Installation
1300
1301
1302
1303
Leginon sessions can be catagorized into different projects. The projects are created
1304
and viewed trhough a set of web tools. To access them, install the tools on the web server.
1305
The same project management tools are also used to create and track appion processing
1306
databases. If you want to use our processing pipeline, Appion, in the future, you must
1307
install this.
1308
1309
1310
h2. Prerequisites for project tools
1311
1312
1313
1314
installed dbemtools and its prerequisites
1315
1316
1317
1318
1319
h2. project tools
1320
1321
1322
Current release: project 1.2.
1323
1324
1325
1326
1327
*  Download project tools from "http://emg.nysbc.org/software/dbemtools":http://emg.nysbc.org/software/dbemtools.
1328
1329
1330
*  Unpack the package in your [webdirectory]
1331
1332
1333
1334
<pre>&gt;cd [webdirectory]			#/var/www/html in this example
1335
[webdirectory]&gt;tar zxvf project_1_2.tgz</pre>
1336
1337
1338
1339
*  Go to the uncompressed directory
1340
project_1_2.<programlisting>> cd project_1_2</pre>
1341
1342
1343
* Copy config.php.template to <emphasis role="bold">config.php</emphasis>
1344
1345
1346
1347
<pre>> cp config.php.template to config.php</pre>
1348
1349
1350
1351
* Edit <emphasis role="bold">config.php</emphasis>: You need to insert these MySQL
1352
parameters in project tool's config.php
1353
1354
1355
1356
<pre>// --- Project Configuration --- //
1357
1358
// --- Leginon database config
1359
$DBEM_PATH="../dbem_1_5_1/";
1360
1361
$DB_HOST  = "[your database host]";
1362
$DB_USER  = "usr_object";
1363
$DB_PASS  = "";
1364
$DB   = "<link linkend="db_example_names">dbemdata</link>";
1365
1366
// --- Project database config
1367
$PROJECT_DB_HOST = "[your database host]";
1368
$PROJECT_DB_USER = "<link linkend="db_example_names">usr_object</link>";
1369
$PROJECT_DB_PASS = "";
1370
$PROJECT_DB = "<link linkend="db_example_names">projectdata</link>";
1371
</pre>
1372
1373
1374
1375
*  Test the set-up by visiting http://yourhost/project_1_2
1376
1377
1378
1379
1380
1381
1382
1383
1384
1385
1386
h2.  Other Tools
1387
1388
1389
This section includes tools that may help with Leginon development.
1390
1391
1392
h2.  phpMyAdmin
1393
1394
1395
1396
1397
1398
*  Download phpMyAdmin from Yast2 or "www.phpmyadmin.net":http://www.phpmyadmin.net
1399
*  Download phpMyAdmin into the directory where the web server has been
1400
installed.
1401
1402
1403
1404
1405
1406
1407
*  Check phpMyAdmin installation:
1408
1409
1410
1411
<pre>&gt;rpm -qa |grep -i phpmyadmin </pre>
1412
1413
1414
1415
*  Install from tarball:
1416
1417
1418
1419
<pre>> cd [webdirectory]
1420
[webdirectory]&gt;tar zxvf phpMyAdmin-2.x.tar.gz</pre>
1421
1422
1423
1424
or Install with yum if availabe:
1425
1426
1427
1428
<pre>> yum install phpMyAdmin</pre>
1429
1430
1431
1432
*  Create phpMyAdmin configuration file called config.inc.php from a
1433
sample.
1434
1435
1436
1437
<pre>[webdirectory]&gt;cd phpMyAdmin
1438
(if you install with yum, the program directory is /usr/share/phpMyAdmin)
1439
1440
> cp config.sample.inc.php config.inc.php
1441
</pre>
1442
1443
1444
1445
*  Make sure these are set correctly in <emphasis role="bold"
1446
>config.inc.php</emphasis>. Note that these are for MySQL which we set earlier.
1447
You can set specific user, but it is safer just leave it blank.
1448
1449
1450
1451
<pre>
1452
$cfg['Servers'][$i]['host']            = '[your host]';
1453
$cfg['Servers'][$i]['port']            = '';
1454
$cfg['Servers'][$i]['socket']          = '';
1455
$cfg['Servers'][$i]['connect_type']    = 'tcp';
1456
$cfg['Servers'][$i]['extension']       = 'mysql';
1457
$cfg['Servers'][$i]['compress']        = FALSE;
1458
$cfg['Servers'][$i]['controluser']     = '';
1459
$cfg['Servers'][$i]['auth_type']     = 'http';
1460
$cfg['Servers'][$i]['user']          = '';
1461
$cfg['Servers'][$i]['password']      = '';
1462
</pre>
1463
1464
1465
1466
*  For additional security, you may restrict mysql root access
1467
1468
1469
1470
<pre>
1471
$cfg['Servers'][$i]['AllowRoot']     = FALSE;
1472
</pre>
1473
1474
1475
1476
*  If you want to access phpMyAdmin from another computer, you can add it to its
1477
web access configuration file found as /etc/httpd/conf.d/phpMyAdmin.conf in a
1478
typical installation
1479
1480
1481
1482
<pre>
1483
&lt;Directory /usr/share/phpMyAdmin/>
1484
order deny,allow
1485
deny from all
1486
allow from 127.0.0.1
1487
allow from YOUR_IP_ADDRESS
1488
&lt;/Directory>
1489
</pre>
1490
1491
1492
1493
1494
1495
1496
1497
1498
1499
1500
1501
1502
1503
h2.  Windows installation
1504
1505
1506
1507
h2.  Processing-side Leginon Windows Installation
1508
1509
1510
1511
h2.  Install Python and Support Packages (Note that python 2.5 must be used):
1512
1513
1514
1515
This list does not include pyton XML module because it is included in the python
1516
package for window.
1517
1518
|_.Name:|_.Download site:|
1519
|Python 2.5*|"http://www.python.org":http://www.python.org|
1520
|Python for Windows extension (pywin32) |"http://sourceforge.net/projects/pywin32/":http://sourceforge.net/projects/pywin32/|
1521
|wxPython 2.5.2.8 or newer|"http://www.wxpython.org":http://www.wxpython.org|
1522
|MySQL Python client 1.2 or newer|"http://sourceforge.net/projects/mysql-python":http://sourceforge.net/projects/mysql-python|
1523
|Python Imaging Library (PIL) 1.1.4 or newer|"http://www.pythonware.com/products/pil/":http://www.pythonware.com/products/pil/|
1524
|NumPy 1.0b5 (tested, others may work)|"http://www.scipy.org":http://www.scipy.org|
1525
|SciPy 0.5.1 or newer|"http://www.scipy.org":http://www.scipy.org|
1526
|Tortoise SVN client|"http://tortoisesvn.tigris.org":http://tortoisesvn.tigris.org|
1527
1528
1529 1 Eric Hou
1530 11 Amber Herold
1531
*Python 2.5 is the only python version that we have compiled numExtension. libCV and
1532
comarray in. Therefore no other python version works for now.
1533
1534
1535
Execute the installer file and follow the directions.
1536
1537
1538
1539
1540
h2. Packages required from NRAMM
1541
1542
1543
1544
Here are the packages you need to install with python installer
1545
1546
|_.Name:|_.Purpose:|
1547
| Leginon|modular TEM image acquisition|
1548
| pyami|general functions|
1549
| sinedon|Leginon/database interaction|
1550
| pyScope|microscope control and monitoring|
1551
| ImageViewer|image viewing for tomography|
1552
1553
1554
1555
1556
Because numextension and libCV requires extra compilers, we have created window
1557 12 Amber Herold
installer for them for python 2.5 and made them available through "http://www.leginon.org/":http://www.leginon.org/|_.Downloadfile Name|_.Installed Python Package File|_.Purpose:|
1558 11 Amber Herold
|NumExtension-1.2.0.win32-py2.5.exe|numextension.pyd|c extension for numerical processing|
1559
|libCV-0.2.win32-py2.5.exe|libCV.pyd|small c library of algorithm from computer vision field|
1560
1561
1562
1563
1564
1565
1566
h2. Check out SVN Source Files from the depository
1567
1568
1569
1570
Use your mouse to do the following
1571
* Create Leginon-1.6-ALL directory somewhere at your convenience
1572
1573
1574
* Change directory into Leginon-1.6-ALL
1575
1576
1577
* Right-click the mouse botton in this directory window and select Tortoise svn
1578
Checkout in the menu:<graphic
1579
fileref="http://emg.nysbc.org/software/leginon/images/images/svnmenu.png"
1580
/>
1581
1582
1583
* Set up svn checkout window like this for <link linkend="windows_packages">EACH
1584
of the leginon packages</link>.<graphic
1585
fileref="http://emg.nysbc.org/software/leginon/images/images/svnco.png"/>
1586 1 Eric Hou
1587 11 Amber Herold
1588
1589
1590
1591
1592
1593
h2. Install the packages you downloaded from NRAMM svn depository
1594
1595
1596
1597
1598
1599
* Start a command line Window from Start Menu
1600
1601
1602
* Install the package in each folder with commands such as
1603
<pre>cd Your_Download_Place\Leginon-1.6-ALL\leginon
1604
c:\\python25\python.exe setup.py install</pre>
1605
1606
1607
1608
1609
1610
1611
1612
1613
h2. Download the two Window Installer Files from Leginon website
1614
1615
1616
1617 12 Amber Herold
"http://www.leginon.org/":http://www.leginon.org/h2. Install individual packages
1618 11 Amber Herold
1619
1620
1621
Excute the installer files and follow the instruction.
1622
1623
1624
1625
1626
h2.  Mapping Drives:
1627
1628
1629
1630
If you plan to run Leginon directly on the Windows machine, such as in <link
1631
linkend="config_C">configuration C</link>, and your data files are served through a
1632
Samba server on a Linux machine, you will need to map the network drive. For example, if
1633
your Samba server has a hostname your_smbserver, and you have set up a share called
1634
[your_share_point] which points to /your_data_path/ and leginon data will be saved under a
1635
folder in /your_data_path/leginon/
1636
1637
1638
1639
1640
*  Start, My Computer
1641
1642
1643
*  Tools menu, Map network drive
1644
1645
1646
*  Use an unmapped drive such as Z:
1647
1648
Enter shared path in Windows format
1649
as<programlisting>\\your_smbserver\your_share_point</pre>
1650
1651
1652
*  Add the drive and the Linux path to leginon.cfg on the Windows machine
1653
as<programlisting>[Drive Mapping]
1654
Z:/your_data_path</pre>
1655
1656
1657
*  Add image path to leginon.cfg on the Windows machine in Linux format
1658
as<programlisting>[Images]
1659
path:/your_data_path/leginon</pre>
1660
1661
1662
1663
1664
1665
1666
1667
1668
h2.  Configure leginon.cfg:
1669
1670
1671
Follow instruction in "<link linkend="leginon_cfg">Configure leginon.cfg</link>" in
1672
the section for Linux installation but note the location of the configuration files
1673
follows. In addition, if the storage disk is mapped onto the Windows PC as drive Z, this
1674
mapping should be included in leginon.cfg. See above.
1675
1676
1677
1678
1679
*  Configurations for all users
1680
1681
1682
1683
<pre> &lt;Python directory&gt;\Lib\site-packages\Leginon\config\leginon.cfg</pre>
1684
1685
1686
Example:
1687
<pre> C:\Python25\Lib\site-packages\Leginon\config\leginon.cfg</pre>
1688
1689
1690
*  Configurations for individual users
1691
1692
1693
1694
<pre> &lt;Home directory&gt;\leginon.cfg</pre>
1695
1696
1697
Example:
1698
<pre> C:\Documents and Settings\Leginon User\leginon.cfg </pre>
1699
1700
1701
*  A skeleton (default) configuration file is available:
1702
1703
1704
1705
<pre> C:\Python25\Lib\site-packages\Leginon\config\default.cfg</pre>
1706
1707
1708
1709
1710
1711
1712
1713
1714
1715
h2.  Configure sinedon.cfg:
1716
1717
1718
Sinedon is designed to be able to interact with multiple databases.
1719
1720
Follow instruction in "<link linkend="sinedon_cfg">Configure sinedon.cfg</link>" in
1721
the section for Linux installation but note the location of the configuration files
1722
follows.
1723
1724
1725
1726
1727
* For all users, put sinedon.cfg with the installed package
1728
as<pre>C:\Python25\Lib\site-packages\sinedon\sinedon.cfg</pre>
1729
1730
1731
*  Your home directory on
1732
Windows:<pre>C:\Documents and Settings\your_name&gt;</pre>
1733
1734
1735
* the skeleton sinedon configuration file
1736
is<pre>C:\Python25\Lib\site-packages\sinedon\examples\sinedon.cfg</pre>
1737
1738
1739
1740
1741
1742
1743
1744
1745
h2.  Create Leginon and Leginon Client shortcut in Start menu menu under Leginon
1746
1747
1748
1749
This instruction refers to Windows XP
1750
1751
1752
1753
1754
* Go to C:\Documents and Settings\All Users\Start Menu\Programs\ and create a new
1755
folder named Leginon.
1756
1757
1758
* In another window, go
1759
to<programlisting>C:\Python25\Lib\site-packages\Leginon</pre>
1760
1761
1762
* Create a shortcut from start-leginon.py as Leginon and a shortcut from
1763
launcher.py as Leginon Client.
1764
1765
1766
* Move the two shortcuts into
1767
<programlisting>C:\Documents and Settings\All Users\Start Menu\Programs\Leginon</pre>
1768
1769
1770
1771
1772
1773
1774
1775
1776
h2.  Additional Software (Optional):
1777
1778
1779
TightVNC (http://www.tightvnc.com)
1780
1781
1782
1783
1784
1785
1786
1787
1788
h2. Database server Windows Installation
1789
1790
1791
1792
h2. We do not do this at NRAMM. Please follow the instruction in Linux installation and
1793
modify it for Windows at your own risk.
1794
1795
1796
1797
For a good Windows specific instruction for general PHP configuration with MySQL for
1798
Apache 2 in Windows, try http://www.artfulsoftware.com/php_mysql_win.html.
1799
1800
1801
1802
1803
1804
1805
1806
1807
h2.  Additional installation on the microscope computer:
1808
1809
1810
1811
The full leginon and its supporting packages need to be installed on the Windows computer
1812
controlling the microscope. Additional programs are required for communication with the CCD
1813
and extra functions for Tecnai scripting not supported as default by FEI Tecnai
1814
microscopes
1815
1816
1817
h2.  Required supporting programs for the CCD camera from camera makers
1818
1819
1820
1821
Install and register the following programs for CCD cameras from the two makes:
1822
1823
|_.Camera Make:|_.File:|
1824
| Gatan|TecnaiCCD.dll|
1825
| Tietz|CAMC4.exe*|
1826
1827
1828
1829
1830
* We have experienced slowness of the CAMC4.exe comes with later version Tecnai TUI/TIA.
1831
Replacing it with an earlier version of CAMC4.exe resolved the problem.
1832
1833
1834
1835
1836
h2. Additional Package required from NRAMM for Gatan camera or camera that uses TIA
1837
1838
1839
1840
For Gatan Camera or FEI Eagle Camera that uses TIA, comarray package need to be install
1841
with python
1842
1843
|_.SVN Package Name|_.Installed Python Package Name|_.Reason for update:|
1844
|comarray|comarray|com module output conversion to array|
1845
1846
1847
1848
1849
1850
1851
* <link linkend="window_svn">Check out the comarray package</link> from svn
1852
depository with your SVN client
1853
1854
<programlisting>cd Leginon-1.6-ALL
1855
svn co http://emg.nysbc.org/svn/comarray/branches/leg1.6 comarray</pre>
1856
1857
* Start a command line Window from Start Menu
1858
1859
1860
* Install the package with commands such as
1861
<pre>cd Your_Download_Place\Leginon-1.6-ALL\comarray
1862
c:\\python25\python.exe setup.py install</pre>
1863
1864
1865
1866
1867
1868
1869
1870
1871
h2.  Supporting programs for film exposure
1872
1873
1874
1875
Install the following if you need film exposure on FEI Tecnai TEM through Leginon,
1876
available through FEI. Please contact Max Otten: <email>mto@feico.com</email> and request
1877
for adaexp.exe that works with your version of Tecnai user interface program.
1878
1879
|_.Name:|_.File:|
1880
| exposure adaptor|adaexp.exe|
1881
1882
1883
1884
1885
1886
1887
h2.  Register adaexep.exe
1888
1889
1890
1891
*  From the command prompt:
1892
1893
<pre>adaexp.exe /regserver</pre>
1894
1895
*  From the program start menu under pyScope or
1896
C:\Python25\Lib\site-packages\pyScope\
1897
1898
<pre>updatecom.py</pre>
1899
1900
1901
1902
1903
1904
1905
1906
h2. Modify instruments.cfg:
1907
1908
1909
1910
Sinedon now have full control of database interaction, therefore, the database
1911
configuration in leginon.cfg is no longer needed.
1912
1913
1914
1915
1916
* Modify the file instruments.cfg in the installed pyScope directory to that for
1917
your microscope and camera. For example, if your microscope uses Tecnai Scripting
1918
Interface and you have a Gatan camera that you interface through
1919
DigitalMicrograph:
1920
1921
1922
1923
<pre>[tem]
1924
class: tecnai.Tecnai
1925
[camera]
1926
class: gatan.Gatan</pre>
1927
1928
1929
1930
The file contains other examples of microscope and camera drivers that we
1931
distribute from NRAMM.
1932
1933
1934
1935
1936
1937
1938
1939
1940
h2. Run updatecom.py
1941
1942
1943
1944
From a command line window:
1945
1946
1947
1948
<pre>cd C:\python25\Lib\Site-Packages\pyScope
1949
C:\python25\python.exe updatecom.py</pre>
1950
1951
1952
1953
This should generate a few files, including tecnaicom.py, gatancom.py and tietzcom.py,
1954
in the same directory.
1955
1956
1957
1958
1959
h2. Additional setup on Tietz PXL camera (optional)
1960
1961
1962
1963
The actual Tietz PXL camera dimension is slightly larger than 2048 x 2048. If you'd like
1964
to take the images using the maximum of 2048 x 2048 pixels, you may modify the function that
1965
get camera dimension in tietz.py of pyScope package.
1966
1967
1968
*  Go to C:\Python25\Lib\site-packages\pyScope\
1969
1970
1971
*  Edit tietz.py with a plain text editor
1972
1973
1974
*  Find the lines:
1975
1976
1977
1978
<pre> def getCameraSize(self):
1979
# {'type': dict, 'values': {'x': {'type': int}, 'y': {'type': int}}}}
1980
x = self._getParameterValue('cpTotalDimensionX')
1981
y = self._getParameterValue('cpTotalDimensionY')
1982
return {'x': x, 'y': y}
1983
</pre>
1984
1985
1986
1987
*  Change the last line to:
1988
1989
1990
1991
<pre>    return {'x': 2048, 'y': 2048}</pre>
1992
1993
1994
1995
1996
1997
1998
1999
h2.  Additional Software (Optional):
2000
2001
2002
TightVNC (http://www.tightvnc.com) if you get tired of going into the microscope room
2003
just to open the column valves.
2004
2005
2006
2007
2008
2009
2010
2011
2012
h2.  What is next
2013
2014
2015
2016
h2.  Perform Administration Setup
2017
2018
2019
See Next chapter on <link linkend="addusers">Leginon Administration Tools</link>.
2020
2021
2022
2023
2024
2025
h2.  Create a test project
2026
2027
2028
See the chapter on <link linkend="project">Project Management Tools</link>.
2029
2030
2031
2032
2033
h2.  Perform Microscope Setup and Test run
2034
2035
2036
See the chapter on <link linkend="runleg_chapter">Start Leginon</link>.
2037
2038
2039
2040
2041
2042
2043
h2.  Backup Practices
2044
2045
2046
2047
h2.  Ideal setup:
2048
2049
2050
2051
h2.  Regular computer backup
2052
2053
2054
2055
To protect your program installation.
2056
2057
2058
2059
2060
h2.  Full backup of the databases every night
2061
2062
2063
2064
The database is the metadata generated by Leginon named "dbemdata" in the installation
2065
example.
2066
2067
2068
2069
2070
h2. Full/Incremental/differential backup of the images
2071
2072
2073
2074
The image files located in [your storage disk] should be backup in full at least once
2075
a month, and nightly in differential or incremental mode.
2076
2077
2078
2079
2080
2081
2082
h2.  Current NRAMM setup:
2083
2084
2085
2086
Because our tape backup system is not on-site and the data have to transfer over slow
2087
network to perform the task, we have not been able to backup in the ideal setting. Here is
2088
what we currently do:
2089
2090
2091
h2.  Regular computer backup
2092
2093
2094
2095
TSRI Research Computing provides regular back up service that does full backup of the
2096
computers every four weeks and incremental backup every night. The full backup expires in
2097
4 months and the incremental backup one month. We do this for the computers running
2098
Leginon main program, the database server, as well as the web server.
2099
2100
2101
2102
2103
h2.  Regular backup &amp; tar of the databases every night
2104
2105
2106
2107
On top of the TSRI backup, we also tar the database everynight and store it on the
2108
institutional tape library.
2109
2110
2111
2112
2113
h2.  rsync to a tape library of the image storage disk
2114
2115
2116
2117
As our image storage disks total 15T byte, we currently use rsync function to update a
2118
copy on our institutional tape library. It creates new files, updates old files, but does
2119
not remove old files on the tape if it is removed on the storage disk. We do not think
2120
this is the best solution since it is not possible to retrieve old files that have been
2121
modified on a later time.