Appion Home » History » Revision 57
Revision 56 (Neil Voss, 05/26/2010 11:26 AM) → Revision 57/101 (Neil Voss, 05/26/2010 11:29 AM)
h1. Appion Main Page
!>http://lh5.google.com/vossman77/RzJbEE3GhuI/AAAAAAAAASY/SSaOA7FZKwI/nramm.jpg?imgdl=1!
h2. Overview
Appion is a "pipeline" for semi-automated three-dimensional electron microscopy starting from raw images to complete three-dimensional models.
It consists of a web based user interface linked to a set of python scripts that control several underlying processing packages. These include EMAN, Spider, findem, ACE, and Chimera. All data input and output is managed by MySQL databases. The ultimate goal is to have all control of the processing pipeline managed from the web based user interface and all output from the processing presented using web based viewing tools. These notes are provided as a rough guide to using the pipeline but are not guaranteed to be up to date or accurate.
h2. Contact information:
Please email _appion@scripps.edu_ with any questions.
h2. Software Installation:
see [[Complete Installation|Complete Appion Installation]]
h2. User Guide:
see [[Appion User Guide|Appion User Guide]]
h2. Publications:
h3. Primary Publication to Cite:
* Lander, G.C.; Stagg, S.M., Voss, N.R., Cheng, A., Fellmann, D., Pulokas, J., Yoshioka, C., Irving, C., Mulder, A., Lau, P.W., _et al._ (2009). "Appion: an integrated, database-driven pipeline to facilitate EM image processing.". Journal of Structural Biology 166: 95-102. "PMID 19263523":http://www.ncbi.nlm.nih.gov/pubmed/19263523.
h3. Additional References:
* Voss, N.R.; Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, _et al._ (2010). "A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.". Journal of Structural Biology 169 (3): 389-98. "PMID 20018246":http://www.ncbi.nlm.nih.gov/pubmed/20018246.
* Stagg, S.M.; Lander GC, Quispe J, Voss NR, Cheng A, Bradlow H, Bradlow S, Carragher B, Potter CS (2008). "A test-bed for optimizing high-resolution single particle reconstructions". Journal of Structural Biology 163 (1): 29-39. "PMID 18534866":http://www.ncbi.nlm.nih.gov/pubmed/18534866.
h2. Software Availability and Licensing Information
Appion is released under the "Apache License, Version 2.0":http://www.apache.org/licenses/LICENSE-2.0
View an [[myami code diagram|overview of the Appion code]] base found in the "myami" repository.
h2. Youtube videos:
* "An Introduction to Appion":http://www.youtube.com/watch?v=Wlxt_4yJKgA
* "TiltPicker Demonstration":http://www.youtube.com/watch?v=z7BqGJczmjU
---