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Appion Processing » History » Version 35

Anke Mulder, 06/14/2010 12:14 PM

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h1. Appion Processing
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## [[Terminology]]
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## [[Common Workflow]]
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### [[Step by Step Guide]]
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### [[Quality Assessment]]
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## [[Processing Cluster Login]]
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## [[Particle Selection]] 
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### [[Template Picking]]
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### [[Dog Picking]]
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### [[Manual Picking]] 
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### [[Repeat from other session]]
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### [[Random Conical Tilt Picking]]
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**** [[Align and Edit Tilt Pairs]]
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**** [[Auto Align Tilt Pairs]]
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## [[CTF Estimation]] 
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### [[Ace Estimation]]
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### [[Ace 2 Estimation]]
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### [[CtfFind Estimation]]
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### [[Repeat from other session]]
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## [[Stacks]] 
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### [[Stack creation]]
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### [[more stack tools]]
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#### [[Combine Stacks]]
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#### [[View Stacks]] 
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***** [[Filter by MeanSTDev]]
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***** [[Center Particles]]
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***** [[Sort Junk]]
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***** [[Create Substack]]
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#### [[Alignment SubStacks]]
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#### [[ Jumpers SubStack]]
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#### [[Convert Stack into Particle Picks]]
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## [[Particle Alignment]] 
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### [[Run Alignment]]
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**** [[Xmipp Maximum Likelihood Alignment]]
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**** [[Spider Reference-free Alignment]]
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**** [[Spider Reference-based Alignment]]
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**** [[Ed's Iteration Alignment]]
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**** [[Xmipp Reference-based Maximum Likelihood Alignment]]
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### [[Run Feature Analysis]]
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### [[Run Particle Clustering]]
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## [[Ab Initio Reconstruction]]
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### [[RCT Volume]] 
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### [[OTR Volume]] 
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### [[EMAN Common Lines]] 
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### [[IMAGIC Angular Reconstitution]] 
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## [[Refine Reconstruction]] 
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### [[EMAN Refinement]]
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### [[Frealign Refinement]]
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### [[SPIDER Refinement]]
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### [[IMAGIC Refinement]]
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## [[Tomography]]
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### [[Align tilt series]]
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### [[Create full tomogram]]
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### [[Upload tomogram]]
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### [[Create tomogram subvolume]]
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### [[Average subvolumes]]
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## [[Import Tools]] 
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### [[PDB to Model]]
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### [[EMDB to Model]]
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### [[Upload Particles]]
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### [[Upload Template]]
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### [[Upload Model]]
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### [[Upload More Images]]
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### [[Upload Stack]]
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## [[Image Assessment]] 
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### [[Web Img Assessment]]
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### [[Multi Img Assessment]]
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### [[Run Image Rejector]]
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## [[Region Mask Creation]]
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### [[Manual Masking]] 
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## [[Synthetic Data]] 
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### [[Synthetic Dataset Creation]]
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!http://emg.nysbc.org/attachments/282/Picture_83.png!
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!http://emg.nysbc.org/attachments/277/Picture_81.png!
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[[Appion and Leginon Database Tools|< Appion and Leginon Database Tools]]
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