Appion

h1. Appion User Guide

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Appion is a "pipeline" for single particle reconstruction integrated with Leginon data acquisition. It consists of a web based user interface linked to a set of python scripts that control several underlying processing packages. These include EMAN, Spider, findem, ACE, and Chimera. All data input and output is managed by MySQL databases. The ultimate goal is to have all control of the processing pipeline managed from the web based user interface and all output from the processing presented using web based viewing tools. These notes are provided as a rough guide to using the pipeline but are not guaranteed to be up to date or accurate.

Appion users usually start off at a web page that presents them with a range of options for processing, reconstruction, analysis. This may have the following appearance:

The user can select to proceed with any of the steps but some of these may be dependent on earlier steps. For example a stack cannot be made until particles have been selected. After any of the steps has been run the user can chose to view the results by clicking on the "completed" or "available" labels. 

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# [[Appion_scripts]]

# [[Appion_output]]

Developed by:

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