Average tomogram subvolumes » History » Revision 6
Revision 5 (Anchi Cheng, 11/09/2010 06:56 PM) → Revision 6/7 (Anchi Cheng, 11/09/2010 07:00 PM)
h1. Average tomogram subvolumes Subvolume averaging implemented here aligns the 3D subvolumes using # The 2D alignment of the particles picked from the Z-projection of the full tomogram for xy plane alignment # Center of mass of the central slice of each subvolume in z direction. h2. General Workflow: # Particles need to be picked on the Z-projection images, # A [[Stacks|stack made]] from these picked particles # The stack need to be [[Particle_Alignment|align and classified]] in 2D # [[Select 2D Classes]] to [[Create [Create Substack|create a substack]] # [[Create tomogram subvolume]] using the substack so that the alignment parameters are linked. # Select "Average subvolumes" in Appion sidebar under Tomography submenu. # Choose the substack # Enter runname (The directory where it is saved is automatically determined now) h2. Notes, Comments, and Suggestions: ______ [[Create tomogram subvolume|< Create Tomogram Subvolumes]] ______