CTF Estimation » History » Revision 5
Revision 4 (Anke Mulder, 05/18/2010 07:01 PM) → Revision 5/18 (Anke Mulder, 05/18/2010 07:05 PM)
h1. CTF Estimation Image formation in Regardless of the method eventually utilized for 3D reconstruction, a good starting point for single particle EM investigations is distorted by modulation 2D alignment and classification of a contrast transfer function (CTF). the dataset. Distortion depends on This type of analysis intimately acquaints the physical parameters scientist with the types of the microscope, such as keV particles, distribution of views, and lens abberations. Correcting for these aberrations is done by comparing the experimentally observed power spectral density (PSD) relative amount of EM images to a theoretically generated CTF. "junk" contained in the dataset. h2. Available CTF Procedures: # [[Ace Estimation]] # [[Ace 2 Estimation]] # [[CtfTilt Estimation]] h2. Appion SideBar Snapshot: !http://emg.nysbc.org/attachments/196/Picture_85.png! h2. Appion Composite Output Snapshot: !http://emg.nysbc.org/attachments/197/Picture_84.png! [[Particle Selection|< Particle Selection]] | [[Stacks|Create Particle Stack >]] ______