Appion

h1. Create full tomogram

!http://emg.nysbc.org/attachments/273/Picture_71.png!

h2. General Workflow:

# Select the tiltseries for which a tomogram is to be calculated.

# Select the alignment run from align tilt series to be used.

# Use the radio button to select Protomo Refinement or IMOD shift-only alignment.

# To submit the job to the cluster click the "Align Tilt Series" button.  Alternatively, click on "Just Show Command" to obtain a command that can be pasted into a UNIX shell.

!http://emg.nysbc.org/attachments/274/Picture_74.png!

# If your job has been submitted to the cluster, a page will appear with a link "Check status of job", which allows tracking of the job via its log-file. This link is also accessible from the "1 running" option under the "Aliogn tilt series" submenu in the appion sidebar.

# Now click on the "1 Complete" link under the "Align tilt series" submenu. This opens a summary of all tilt series alignment runs that have been completed for this dataset.

!http://emg.nysbc.org/attachments/266/Picture_61.png!

# Clicking on the "alignrun id" opens a summary page for the alignment cycles that were run.

# Clicking on the "refine cycle" number opens a report page for that cycle including input parameters and directory paths.  Clicking on "Alignment Movie" opens a new web-browser with a movie of the aligned tilt series.

# The same alignment cycle can be repeated by clicking "Repeat Last Aligner Iteration" or another initiated by clicking "Setup Next Aligner Iteration".

# In order to [[Create full tomogram|calculate a tomogram]] from the aligned tiltseries, click on the "Create full tomogram" link in the Tomography menu on the Appion Sidebar.

!http://emg.nysbc.org/attachments/272/Picture_70.png!

h2. Notes, Comments, and Suggestions:

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[[Tomography|< Tomography]] | [[Create full tomogram|Create Full Tomogram >]]

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