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Create full tomogram » History » Revision 9

Revision 8 (Anke Mulder, 06/03/2010 06:35 PM) → Revision 9/11 (Anke Mulder, 06/03/2010 06:37 PM)

h1. Create full tomogram 

 !http://emg.nysbc.org/attachments/273/Picture_71.png! 

 h2. General Workflow: 

 # Select the tiltseries for which a tomogram is to be calculated. 
 # Select the alignment run from [[Align tilt series]] to be used. 
 # Check the run name and enter a description. 
 # To submit the job to the cluster click the "Create Full Tomogram" button.    Alternatively, click on "Just Show Command" to obtain a command that can be pasted into a UNIX shell. 
 !http://emg.nysbc.org/attachments/274/Picture_74.png! 
 # If your job has been submitted to the cluster, a page will appear with a link "Check status of job", which allows tracking of the job via its log-file. This link is also accessible from the "1 running" option under the "Create full tomogram" submenu in the appion sidebar. 
 # Now click on the "1 Complete" link under the "Create full tomogram" submenu. This opens a summary of all tomograms that have been calculated for this dataset. 
 !http://emg.nysbc.org/attachments/275/Picture_77.png! 
 # Clicking on the "id" "alignrun id" opens a summary page for the calculated tomogram alignment cycles that includes were run. 
 # Clicking on the "refine cycle" number opens a report page for that cycle including input parameters and file directory paths.    Clicking on "Alignment Movie" opens a new web-browser with a movie of the aligned tilt series. 
 # The same alignment cycle can be repeated by clicking "Repeat Last Aligner Iteration" or another initiated by clicking "Setup Next Aligner Iteration". 
 # In order to [[Upload [[Create full tomogram|upload this tomogram|calculate a tomogram]] for further processing, from the aligned tiltseries, click on the "Upload "Create full tomogram" link in the Tomography menu on the Appion Sidebar. 
 !http://emg.nysbc.org/attachments/276/Picture_80.png! 

 h2. Notes, Comments, and Suggestions: 


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 [[Align tilt series|< Align Tilt Series]] | [[Upload full tomogram|Upload Full Tomogram >]] 

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