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Create tomogram subvolume » History » Revision 6

Revision 5 (Anke Mulder, 06/10/2010 04:37 PM) → Revision 6/16 (Anke Mulder, 06/10/2010 04:39 PM)

h1. Create tomogram subvolume 

 In order to upload subvolumes of a tomogram, the user first needs to use the [[particle selection]] tool to pick which portions of the full tomogram to upload.    The protocol for this step, as well as creation of the subvolume, is outlined below. 

 h2. General Workflow: 

 # In the Appion Sidebar, select the "manual picking" option under the "Particle Selection" submenu. 
 # Check the run name and output directory. 
 # Select the "zproj" present from the dropdown menu.    This means that you will be selecting portions of your tomogram using only these images. 
 # Set a diameter for the resulting images (won't affect actual boxing, just display). 
 # Check the commit to database box if desired. 
 # Click the "Just Show Command" button to copy the command into a unix terminal. 
 !http://emg.nysbc.org/attachments/330/Picture_68.png! 


 

 # To [[Average tomogram subvolumes|average multiple tomogram subvolumes]], click on the "Average tomogram subvolumes" link in the "Tomography" submenu on the Appion sidebar. 



 h2. Notes, Comments, and Suggestions: 


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 [[Create full tomogram|< Create Full Tomogram]] | [[Average tomogram subvolumes|Average Tomogram Subvolumes >]] 

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