Create tomogram subvolume » History » Revision 8
Revision 7 (Anke Mulder, 06/10/2010 04:43 PM) → Revision 8/16 (Anke Mulder, 06/10/2010 04:43 PM)
h1. Create tomogram subvolume
In order to upload subvolumes of a tomogram, the user first needs to use the [[particle selection]] tool to pick which portions of the full tomogram to upload. The protocol for this step, as well as creation of the subvolume, is outlined below.
h2. General Workflow:
# In the Appion Sidebar, select the "manual picking" option under the "Particle Selection" submenu.
# Check the run name and output directory.
# Select the "zproj" present from the dropdown menu. This means that you will be selecting portions of your tomogram using only these images.
# Set a diameter for the resulting images (won't affect actual boxing, just display).
# Check the commit to database box if desired.
# Click the "Just Show Command" button.
!http://emg.nysbc.org/attachments/330/Picture_68.png!
# A new window will open with a command that can be copied and then:
# pasted into a unix terminal to run. NOTE: Manual picker cannot be run from the webpage!
!http://emg.nysbc.org/attachments/331/Picture_71.png!
_# Many more steps to come......_
# To [[Average tomogram subvolumes|average multiple tomogram subvolumes]], click on the "Average tomogram subvolumes" link in the "Tomography" submenu on the Appion sidebar.
h2. Notes, Comments, and Suggestions:
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[[Create full tomogram|< Create Full Tomogram]] | [[Average tomogram subvolumes|Average Tomogram Subvolumes >]]
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