Create tomogram subvolume » History » Revision 9
Revision 8 (Anke Mulder, 06/10/2010 04:43 PM) → Revision 9/16 (Anke Mulder, 07/08/2010 05:11 PM)
h1. Create tomogram subvolume In order to upload subvolumes of a tomogram, the user first needs to use the [[particle selection]] tool to pick which portions of the full tomogram to upload. The protocol for this step, as well as creation of the subvolume, is outlined below. h2. General Workflow: # In the Appion Sidebar, select the "manual picking" option under the "Particle Selection" submenu. # Check the run name and output directory. # Select the "zproj" present from the dropdown menu. This means that you will be selecting portions of your tomogram using only these images. # Set a diameter for the resulting images (won't affect actual boxing, just display). # Check the commit to database box if desired. # Click the "Just Show Command" button. !http://emg.nysbc.org/attachments/330/Picture_68.png! # A new window will open with a command that can be copied and then: # pasted into a unix terminal to run. NOTE: Manual picker cannot be run from the webpage! !http://emg.nysbc.org/attachments/331/Picture_71.png! # _# Many more steps to come...... come......_ # To [[Average tomogram subvolumes|average multiple tomogram subvolumes]], click on the "Average tomogram subvolumes" link in the "Tomography" submenu on the Appion sidebar. h2. Notes, Comments, and Suggestions: ______ [[Create full tomogram|< Create Full Tomogram]] | [[Average tomogram subvolumes|Average Tomogram Subvolumes >]] ______