Appion

h1. OTR Volume

h2. *Introduction:*

The orthogonal tilt reconstruction method is an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles (Leschziner & Nogales, 2005). The method involves collecting data at +45° and −45° tilts and only requires that particles adopt a relatively large number of orientations on the grid. One tilted data set is used for alignment and classification and the other set—which provides views orthogonal to those in the first—is used for reconstruction, resulting in the absence of a missing cone.

h2. *OTR Processing in Appion:*

h3. Requirements:

# Having run either auto tilt align or manual tilt align

#