Particle Selection » History » Version 19
Anke Mulder, 06/04/2010 10:37 AM
| 1 | 1 | Amber Herold | h1. Particle Selection |
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| 3 | 4 | Arne Moeller | The first step in single particle analysis is to pick the particles within the micrographs. Basically three main ways exist to do this, all of them are integrated within Appion. Based on the shape of the particle, the prior knowledge and the amount of data collected, the user has to make a decision which approach is the best or use different approaches simultaneously and see which works best. |
| 4 | 1 | Amber Herold | |
| 5 | 18 | Anke Mulder | h2. Available Particle Picking Tools: |
| 6 | 16 | Arne Moeller | |
| 7 | 18 | Anke Mulder | # [[Manual Picking]] |
| 8 | # [[Template Picking]] |
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| 9 | # [[Dog Picking]] |
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| 10 | # [[Random Conical Tilt Picking]] |
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| 11 | ## [[Align and Edit Tilt Pairs]] |
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| 12 | ## [[Auto Align Tilt Pairs]] |
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| 13 | # [[Repeat from other Session]] |
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| 14 | 1 | Amber Herold | |
| 15 | 19 | Anke Mulder | h2. Appion SideBar Snapshot: |
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| 17 | !http://emg.nysbc.org/attachments/283/Picture_84.png! |
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| 19 | 17 | Anke Mulder | h2. Notes, Comments, and Suggestions: |
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| 21 | !http://emg.nysbc.org/attachments/282/Picture_83.png! |
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| 22 | 15 | Arne Moeller | !http://emg.nysbc.org/attachments/277/Picture_81.png! |
| 23 | 17 | Anke Mulder | |
| 24 | 14 | Arne Moeller | |
| 25 | 1 | Amber Herold | ______ |
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| 27 | 2 | Amber Herold | [[Processing Cluster Login|< Processing Cluster Login]] | [[CTF Estimation|CTF Estimation >]] |
| 28 | 1 | Amber Herold | |
| 29 | ______ |