Appion

h1. Reconstruction Parameters

|_.Name|_.a.k.a.|_.Description|_.Used By|_.Required in interface?|_.Notes|

|Mask Radius|mask|raduius of external map in pixels|xmipp, eman, imagic|||

|Inner Radius||inner radius for alignment in pixels|xmipp|||

|imask|Particle inner mask radius|radius of internal mask (in pixels for eman and spider, Angstroms for frealign)|EMAN, frealign, spider|||

|Outer Radius|||xmipp|||

|Particle outer mask radius (RO)|mask|radius from center of particle to outer edge (frealign in angstroms, spider in pixels)|frealign, spider|||

|amask||amask=[r],[threshold],[iter]|eman|||

|MRA - min radius for rotational alignment in pixels|||imagic|||

|MRA - max radius for rotational alignment in pixels|||imagic|||

|firstring||Any pixels this far away from center will not be used|spider|||

|lastring||Only pixels this far away will be used|spider|||

|xysearch||translational search during projection matching will be limited to this many pixels from the center ofthe image|spider|||

|Symmetry Group|sym|ex. c1, c2...|xmipp,eman,frealign,spider,imagic|||

|Number of iterations|iteration number||xmipp|||

|Mask Filename|||xmipp|||

|Angular reconstitution||angular increment of forward projections, ang inc of euler search, fraction of best ordered images to keep|imagic|||

|Angular Sampling Rate|||xmipp|||

|Max angular change|||xmipp|||

|max change offset|||xmipp|||

|Search range for 5d transitional search|||xmipp|||

|Discard % of image with CCF below|||xmipp|||

|Reconstruction Method|||xmipp|||

|Values of lambda for ART|||xmipp|||

|Initial max frequency used by reconstruct fourier|||xmipp|||

|Compute resolution?|||xmipp|||

|Low Pass Filter the reference?|||xmipp|||

|Do not use estimated resolution for low-pass filtering?|||xmipp|||

|Constant to add to the estimated resolution|||xmipp|||

|ang||angular step for projections in degrees|EMAN|||

|angular increments||list of angular increments for projection mapping|spider|||

|MRA - angular increment of forward projections|||imagic|||

|maxshift||max translation during image alignment in pixels|eman|||

|xyshift||particles only allowed to shift this far from center|spider|||

|MRA - max radial shift compared to original images|||imagic|||

|MRA - max radial shift during this iteration|||imagic|||

|hard||hard limit for make3d program|eman|||

|clskeep||=[std dev multipier] how many raw particles discarded for each class average|eman|||

|clsiter||iterative alignmnet to each other|eman|||

|filt3d||applies gaussian low pass filter in pixels [rad]|eman|||

|xfiles||=[mas in kD]|eman|||

|Approximate mass in Kd |||imagic|||

|MSA - percentage of images to ignore when calculating eiganimages|||imagic|||

|shrink||scale down by a factor of [n] before classification|eman|||

|euler2||=[oversample factor]|eman|||

|median||use median value instead of average for each pixel|eman|||

|phscls||use signal to noise ratio weighted phase residual|eman|||

|refine||do subpixel alignment|eman|||

|tree||decimate reference populatiuon|eman|||

|coran||use coran algorithm|eman|||

|eotest||use even odd test|eman|||

|amplitude contrast (WGH)|||frealign|||

|Standard deviation filtering|||fealign|||

|keep||determines which particles are kept for back-projection, -1 is one standard deviation worse than mean|spider|||

|MSA - fraction of final class averages to keep|||imagic|||

|Phase B-factor weighting constant (PBC) |||frealign|||

|B-factor offset (BOFF) |||frealign|||

|Number of randomized search trials (ITMAX) |||frealign|||

|Number of potential matches to refine (IPMAX)|||frealign|||

|Target phase residual (TARGET) |||frealign|||

|Worst phase residual for inclusion (THRESH)|||frealign|||

|Resolution limit of reconstruction (RREC) (in Ångstroms; default Nyquist) |||frealign|||

|lp|filtering|low pass filter in angstroms|spider, imagic|||

|hp|filtering|high pass filter in angstroms|spider, imagic|||

|Lower resolution limit or high-pass filter (RMAX1) (in Ångstroms) |||frealign|||

|Higher resolution limit or low-pass filter (RMAX2) (in Ångstroms; default 2*Nyquist) |||frealign|||

|B-factor correction (RBFACT) (0 = off)|||frealign|||

|Only use CTFFIND values|||frealign|||

|Last particle to use|||frealign|||

|MSA - num class averges to produce from raw images|||imagic|||

|MSA - num factors to use for classification|||imagic|||

|MSA - percentage of worst class members to ignore after classification|||imagic|||

|threed reconstruction||object size, low-pass filter 3d volume|imagic|||

|Center stack prior to alignment |||imagic|||

|Mirror references for alignment |||imagic|||