Upload More Images » History » Revision 3
Revision 2 (Pick-Wei Lau, 05/17/2010 01:23 PM) → Revision 3/11 (Pick-Wei Lau, 05/17/2010 01:42 PM)
h1. Upload More Images The user is able to upload raw micrographs to either existing or new session to be used in appion processing h2. To launch: # The user provides prepares the session name (new or exisiting) as well as a description to the session (if new) # The user has the option to select the origin of the micrographs (e.g. tecnai2 on tietz camera) # If it is a tilt series, the user will need to provide the number of images within the tilt series as well as the tilt information model in the following parameter file # Following this, the user has the option to upload via specifying a parameter file or enter the parameter manually. h3. Specify a Parameter File: # This file has to adhere to the following information: ## complete path and name of micrograph ## pixel size in meters [1.63e-10] ## binning in x [1] ## binning in y [1] ## nominal scope magnification [50000] ## intended defocus in meters [-2e-06] ## high tension in volts [200000] ## (optional) stage alpha tilt in degrees if loading tilt group [55] # The values for each micrographs should be on the same line separated by tabs: > > /home/abc/xxx.mrc 1.63e-10 1 1 50000 -2e-06 200000 55 > > /home/abc/xyz.mrc 1.63e-10 1 1 50000 -2e-06 200000 10 h3. Enter parameters manually: # The user MRC format (user provides the path of the directory containing the images # The user provides the entire file format path) # The user provides a brief description for the pixel size of the micrographs in Angstrom model # The user provides inputs the binning in x # The user provides symmetry for the binning in y model # The user provides inputs the magnification # The user provides resolution of the defocus in micron model # The user provides inputs the high tension of pixel size for the microscope in kV model For a series of untilted images, it might be easier to enter parametes manually (assuming all your micrographs are in the same directory and all the other informations are the same for each image). For tilted images, the user will definiltey need to create a parameter file to incorporate the stage alpha tilt information during upload. h2. Output: # Once the model is uploaded, it will appear viewable under "Upload Model". # The model will be usable for 3D refinement ______ [[Upload Model|< Upload Model]] | [[Upload Stack|Upload Stack >]] ______