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Adding DE-12 to Leginon » History » Version 14

Anchi Cheng, 09/28/2016 07:32 AM

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h1. Installation Guide for DE-12 or DE-20
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h2.  DE-12 is on a computer separated from the microscope
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Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first.
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h2. Extra Package and Installation
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|_.program/package|_.notes|
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|DirectElectronAPI.exe|(Should come with the camera)|
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|"Google protobuf":http://code.google.com/p/protobuf/||
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{{include(portobuf installation instruction)}}
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h2. instruments.cfg
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{{include(Instrument.cfg for DE-12)}}
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height and width should be set according to the model.
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h2. orientation setup
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Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing.
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<pre>
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# frame flipping configuration.
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# default here is for DE-12 orientation on FEI F20
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FRAME_LR_FLIP = True
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# frame rotate configuration. multiple of 90 degrees if needed
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# after the flip.  Direction + is x to -y.
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FRAME_ROTATE = 0
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</pre<
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Known values:
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DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0
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DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1
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h2. Setup
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* Create the folder to store the dose fractionation raw frames.  Default location is D:\\frames.
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* Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server.
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h2. Testing
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h2. Testing with pyscope
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{{include(DE-12 Installation Pyscope Test)}}
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h2. Programs to open before Leginon Client: DE Server
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See how to use DE camera usage in
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* [[DE-12 introduction]] Please read this!!!
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* [[Acquiring summed frame image thru Leginon]]
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* [[DDD raw frame saving|Triggering raw frame saving]]
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* [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive.
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* [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon