Leginon

h1. Installation Guide for DE-12 or DE-20

h2.  DE-12 is on a computer separated from the microscope

Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first.

h2. Extra Package and Installation

|_.program/package|_.notes|

|DirectElectronAPI.exe|(Should come with the camera)|

|"Google protobuf":http://code.google.com/p/protobuf/||

{{include(portobuf installation instruction)}}

h2. instruments.cfg

{{include(Instrument.cfg for DE-12)}}

height and width should be set according to the model.

h2. orientation setup

Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing.

<pre>

# frame flipping configuration.

# default here is for DE-12 orientation on FEI F20

FRAME_LR_FLIP = True

# frame rotate configuration. multiple of 90 degrees if needed

# after the flip.  Direction + is x to -y.

FRAME_ROTATE = 0

</pre>

Known values:

DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0

DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1

h2. Setup

* Create the folder to store the dose fractionation raw frames.  Default location is D:\\frames.

* Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server.

h2. Testing

h2. Testing with pyscope

{{include(DE-12 Installation Pyscope Test)}}

h2. Programs to open before Leginon Client: DE Server

See how to use DE camera usage in

* [[DE-12 introduction]] Please read this!!!

* [[Acquiring summed frame image thru Leginon]]

* [[DDD raw frame saving|Triggering raw frame saving]]

* [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive.

* [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon