Project

General

Profile

Adding DE-12 to Leginon » History » Version 16

Anchi Cheng, 09/28/2016 07:34 AM

1 9 Anchi Cheng
h1. Installation Guide for DE-12 or DE-20
2 1 Anchi Cheng
3 9 Anchi Cheng
h2.  DE-12 is on a computer separated from the microscope
4 1 Anchi Cheng
5 9 Anchi Cheng
Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first.
6 1 Anchi Cheng
7 9 Anchi Cheng
h2. Extra Package and Installation
8
9 12 Anchi Cheng
|_.program/package|_.notes|
10
|DirectElectronAPI.exe|(Should come with the camera)|
11
|"Google protobuf":http://code.google.com/p/protobuf/||
12
13
{{include(portobuf installation instruction)}}
14
15
16 9 Anchi Cheng
17
h2. instruments.cfg
18
19
{{include(Instrument.cfg for DE-12)}}
20
height and width should be set according to the model.
21
22 14 Anchi Cheng
h2. orientation setup
23
24
Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing.
25
26
<pre>
27
# frame flipping configuration.
28
# default here is for DE-12 orientation on FEI F20
29
FRAME_LR_FLIP = True
30
# frame rotate configuration. multiple of 90 degrees if needed
31
# after the flip.  Direction + is x to -y.
32
FRAME_ROTATE = 0
33 15 Anchi Cheng
</pre>
34 14 Anchi Cheng
35 16 Anchi Cheng
*Known values:*
36
37 14 Anchi Cheng
DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0
38
DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1
39
40 9 Anchi Cheng
h2. Setup
41
42
* Create the folder to store the dose fractionation raw frames.  Default location is D:\\frames.
43
* Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server.
44
45 1 Anchi Cheng
h2. Testing
46 7 Anchi Cheng
47 9 Anchi Cheng
h2. Testing with pyscope
48 1 Anchi Cheng
49 10 Anchi Cheng
{{include(DE-12 Installation Pyscope Test)}}
50 1 Anchi Cheng
51 10 Anchi Cheng
h2. Programs to open before Leginon Client: DE Server
52
53
See how to use DE camera usage in
54 11 Anchi Cheng
55
* [[DE-12 introduction]] Please read this!!!
56
* [[Acquiring summed frame image thru Leginon]]
57
* [[DDD raw frame saving|Triggering raw frame saving]]
58
* [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive.
59
* [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon