Aperture selection through tem controller » History » Version 5
Anchi Cheng, 04/29/2020 05:24 PM
1 | 1 | Anchi Cheng | h1. Aperture selection through tem controller |
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3 | This feature allows selection of aperture of TFS microscopes with automated apertures. The current implementation uses AutoIt Scripting and reads the configuration from c:\Program Files (x86)\myami\fei.cfg |
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5 | h2. Setup |
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7 | h3. compile autoit script |
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9 | 5 | Anchi Cheng | See [[AutoIt_program_and_script_compilation]] |
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11 | 1 | Anchi Cheng | The script is pyscope/autoit/ApertureSelection.au3 |
12 | 2 | Anchi Cheng | Use AutoIt suite to compile it. Either 32 or 64-bit version works as far as I know. Save the executable at the place you plan to specify in fei.cfg |
13 | 1 | Anchi Cheng | |
14 | h3. Setup pyscope fei.cfg |
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16 | Copy from pyscope/fei.cfg.template these lines to fei.cfg used on your scope. In standard installation, this should be "c:\Program Files (x86)\myami\fei.cfg" |
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17 | 5 | Anchi Cheng | |
18 | *Note: The current script assumes fei.cfg is in the above-mentioned location. If you put it somewhere else, please change the autoit script to reflect that.* |
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19 | 1 | Anchi Cheng | |
20 | Modify these settings according to your scope. |
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22 | <pre> |
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23 | [aperture] |
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24 | # Disable control of auto apertures if not working or not available |
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25 | # not all versions of microscope software allows auto aperture control |
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26 | # from scripting level. |
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27 | USE_AUTO_APERTURE = False |
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28 | # AutoIt Automation |
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29 | AUTOIT_APERTURE_SELECTION_EXE_PATH = c:\Users\supervisor\Desktop\Anchi\AutoItScripts\ApertureSelection.exe |
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30 | # Does the Titan column has automated c3 aperture ? This affects the id of the |
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31 | # gui button for AutoIt script. |
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32 | HAS_AUTO_C3 = True |
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33 | # Individual aperture selections. If retractable, add open at the end of the list |
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34 | 2 | Anchi Cheng | # List these in the order of what appears in the TUI |
35 | 1 | Anchi Cheng | # Note: can not handle multiple apertures of the same name. |
36 | CONDENSER_2 = 150, 100, 50, 20 |
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37 | OBJECTIVE = 100, 70, 50, open |
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38 | # Optional selected area aperture control. comment or remove the line to deactivate it. |
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39 | SELECTED_AREA = 200,100, 40, 10, open |
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40 | 2 | Anchi Cheng | |
41 | </pre> |
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43 | h2. Testing |
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45 | h3. Testing the AutoIt executable |
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47 | # Open TEM user interface. |
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48 | # Retract the objective aperture. |
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49 | # Double click Autoit executable to run. |
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50 | If this works right, it should insert the 100 um aperture. |
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52 | h3. Testing in with python command from pyscope |
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54 | <pre> |
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55 | from pyscope import fei |
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56 | f=fei.Krios() # Change this to match your instrument class |
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58 | f.getApertureSelections('objective') # This should give you the list you specified in fei.cfg |
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59 | '100', '70', '50' , 'open' |
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61 | f.getApertureSelection('objective') # The returned value should be the current aperture position as a string |
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62 | '100' |
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64 | f.setApertureSelection('objective', '50') # This should insert the 50 um aperture |
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66 | f.retractApertureMechanism('objective') # This should retract the aperture as if you click on the Activate/Deactivate aperture button to disable it. |
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67 | 1 | Anchi Cheng | |
68 | </pre> |
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69 | 3 | Anchi Cheng | |
70 | h2. Use it in Leginon |
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72 | 4 | Anchi Cheng | The most likely usage of this is in [[TEM_Controller|TEM controller]] (aliased as TEM_Remote in MSI application). |