Beam Size Calibration » History » Version 2
Sargis Dallakyan, 06/25/2015 07:49 PM
| 1 | 1 | Anchi Cheng | h1. Beam Size Calibration |
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| 3 | Beam size of FEI microscope can be approximate with a linear function. This calibration determines the coefficient of this linear equation with two points, one where the beam is at cross-over and one at a known size on the main viewing screen (or FluCam on Krios). |
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| 5 | h2. Procedure |
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| 7 | # Select the preset beam size is to be calibrated in "Presets Manager" node and send it to the scope. Most likely this is "en" preset used in the final exposure. |
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| 8 | # Left click on !http://emg.nysbc.org/software/leginon/images/icons/play.png! in the toolbar to start calibration. |
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| 9 | # If C2 aperture size is not specified at the start of the session, the dialog will ask for the value at this point. |
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| 10 | # Put the main screen of the scope down and expand the beam to a known size. The 4 cm diameter circle on FEI scope is a good reference. |
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| 11 | # Enter the size of the beam at this known size in the settings and then click on "Save" in the settings dialog |
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| 12 | # Contract the beam to the cross-over and click on "Save Focused Beam" in the settings dialog. |
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| 13 | # Click "OK" to exit the dialog and save the calibration |
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| 15 | !beam_size_cal_settings.png! |