Calibration without presets » History » Version 1
Amber Herold, 04/22/2010 04:49 PM
| 1 | 1 | Amber Herold | h1. Calibration without presets |
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| 4 | Calibrations can be made without presets although not as conveniently. For each of the |
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| 5 | calibration node, the following need to be done before the calibration or even before |
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| 6 | acquiring a test image. |
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| 10 | h2. Select the TEM and Camera Configuration |
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| 16 | * Leginon/Some Calibraion Node/Toolbar> Click the "Settings" button !http://emg.nysbc.org/software/leginon/images/icons/settings.png!. |
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| 19 | * Leginon/Same Calibration Node/Settings> Override Preset = yes |
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| 22 | * Leginon/Same Calibration Node/Settings/Instrument> TEM = your microscope; CCD |
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| 23 | Camera = your CCD camera |
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| 26 | * Leginon/Same Calibration Node/Camera Configuration> Choose according to your |
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| 27 | need. Usually a highly binned configuration that uses the whole camera. |
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| 30 | * Leginon/Same Calibration Node/Camera Configuration> Click OK to save and close |
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| 31 | the window. |
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| 34 | * Leginon/Pixel Size/Same Calibration Node> Acquire a test image with the camera |
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| 35 | tool!http://emg.nysbc.org/software/leginon/images/icons/acquire.png! to confirrm that the TEM and camera settings are valid. |