Calibration without presets » History » Version 2
Amber Herold, 04/27/2010 10:43 AM
1 | 1 | Amber Herold | h1. Calibration without presets |
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4 | Calibrations can be made without presets although not as conveniently. For each of the |
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5 | calibration node, the following need to be done before the calibration or even before |
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6 | acquiring a test image. |
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10 | h2. Select the TEM and Camera Configuration |
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16 | * Leginon/Some Calibraion Node/Toolbar> Click the "Settings" button !http://emg.nysbc.org/software/leginon/images/icons/settings.png!. |
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19 | * Leginon/Same Calibration Node/Settings> Override Preset = yes |
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22 | * Leginon/Same Calibration Node/Settings/Instrument> TEM = your microscope; CCD |
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23 | Camera = your CCD camera |
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26 | * Leginon/Same Calibration Node/Camera Configuration> Choose according to your |
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27 | need. Usually a highly binned configuration that uses the whole camera. |
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30 | * Leginon/Same Calibration Node/Camera Configuration> Click OK to save and close |
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31 | the window. |
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34 | * Leginon/Pixel Size/Same Calibration Node> Acquire a test image with the camera |
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35 | tool!http://emg.nysbc.org/software/leginon/images/icons/acquire.png! to confirrm that the TEM and camera settings are valid. |
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36 | 2 | Amber Herold | |
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40 | ______ |
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42 | [[Creating Presets for the first time|< Creating Presets for the first time]] | [[Pixel Size Calibration|Pixel Size Calibration >]] |
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44 | ______ |