Leginon

h1. Calibration without presets

Calibrations can be made without presets although not as conveniently. For each of the

calibration node, the following need to be done before the calibration or even before

acquiring a test image.

h2.  Select the TEM and Camera Configuration

*  Leginon/Some Calibraion Node/Toolbar> Click the "Settings" button !http://emg.nysbc.org/software/leginon/images/icons/settings.png!.

*  Leginon/Same Calibration Node/Settings> Override Preset = yes

*  Leginon/Same Calibration Node/Settings/Instrument> TEM = your microscope; CCD

Camera = your CCD camera

*  Leginon/Same Calibration Node/Camera Configuration> Choose according to your

need. Usually a highly binned configuration that uses the whole camera.

*  Leginon/Same Calibration Node/Camera Configuration> Click OK to save and close

the window.

*  Leginon/Pixel Size/Same Calibration Node> Acquire a test image with the camera

tool !http://emg.nysbc.org/software/leginon/images/icons/acquire.png! to confirm that the TEM and camera settings are valid.

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[[Creating Presets for the first time|< Creating Presets for the first time]] | [[Pixel Size Calibration|Pixel Size Calibration >]]

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