Leginon

h1. Do and Do Not in leginon

*  DO NOT use low dose mode unless "Manual" application is used.

*  DO wait for > 1/2 hr, with scope in high mag HM mode, after LM atlas is

acquired.

*  DO check and align microscope in both HM and LM mode well.

*  DO wait for targets to finish processing and Do refresh them on the atlas in "Square

Targeting" Node before selecting and submitting new ones.

*  DO use the "Pause" tool in nodes such as "Square," "Hole," and in Maunal Check window

of "Z Focus" and "Focus"to pause MSI and adjust microscopy parameters directly.

*  DO NOT move the stage at the microscope while Leginon pauses for user verification of

"Exposure Targeting" without using Queuing option on the node. If you do so, click the

"Declare Drift" button " ! " in "Target Adjustment" node.

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[[Getting Help - 2|< Getting Help]] | [[Pausing and Aborting during data collection|Pausing and Aborting during data collection >]]

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