Falcon acquire image » History » Version 1
Anchi Cheng, 07/22/2014 08:09 PM
| 1 | 1 | Anchi Cheng | h1. Acquire images on Falcon II |
|---|---|---|---|
| 2 | |||
| 3 | h2. Preparation in TUI/TIA |
||
| 4 | |||
| 5 | h3. Acquire Gain/Dark References |
||
| 6 | |||
| 7 | Follow FEI's instruction in obtaining Gain/Dark References. If you plan to save frames, make sure you repeat the process with "Store intermediate images" option on. |
||
| 8 | |||
| 9 | h2. Recommended dose rate |
||
| 10 | |||
| 11 | The optimal dose rate is likely between 3 - 3.5 electrons/pixel/frame or 50-60 electron/pixel/s based on available literature of high resolution structures using this camera. |
||
| 12 | |||
| 13 | h2. Preset Recommendation |
||
| 14 | |||
| 15 | An example can be found in [[Pre-MSI_Set-up#Preset-Design-Example-for-Falcon-II-on-Krios]] |