Leginon

h1. Installation on the microscope computer

Only processing-side of Leginon system is needed

h1. Package requirement

h2.  Python and Support Packages (Note that python 2.5 must be used):

This list does not include the python XML module because it is included in the python package for Windows.  You should generally be able to use the most recent versions of these packages that are available from their respective web sites.  Just be sure to always get the version that is compatible with Python 2.5.  If you are having trouble with the most recent version, try to use the specific versions listed here:

|Python 2.5*| "http://www.python.org":http://www.python.org|

|Python for Windows extension (pywin32)| "http://sourceforge.net/projects/pywin32/":http://sourceforge.net/projects/pywin32/|

|wxPython 2.5.2.8 or newer| "http://www.wxpython.org":http://www.wxpython.org|

|MySQL Python client 1.2 or newer| "http://sourceforge.net/projects/mysql-python":http://sourceforge.net/projects/mysql-python|

|Python Imaging Library (PIL) 1.1.4 or newer| "http://www.pythonware.com/products/pil/":http://www.pythonware.com/products/pil/|

|NumPy 1.0b5 (tested, others may work)| "http://www.scipy.org":http://www.scipy.org|

|SciPy 0.5.1 or newer| "http://www.scipy.org":http://www.scipy.org|

*Python 2.5 is the only python version that we have compiled numExtension. libCV and comarray in. Therefore no other python version works for now.

h2. SVN client used for check out from our repository

This required for following this instruction. We have used Tortois SVN client.  Alternatively, you may copy the required NRAMM source files from another computer.

|_.Name:|_.Download site:|

|Tortoise SVN client| "http://tortoisesvn.tigris.org":http://tortoisesvn.tigris.org|

h2.  Required supporting programs for the CCD camera from camera makers

Install and register the following programs for CCD cameras from the two makes.  Most likely, you already have these installed when the camera and TEM software was installed:

|_.Camera Make:|_.File:|

| Gatan|TecnaiCCD.dll|

| Tietz|CAMC4.exe*|

*Note:* We have experienced slowness of the CAMC4.exe comes with later version Tecnai TUI/TIA. Replacing it with an earlier version of CAMC4.exe resolved the problem

h2.  Supporting programs for film exposure

Install the following if you need film exposure on FEI Tecnai TEM through Leginon, available through FEI. Please contact Max Otten: mto@feico.com and request for adaexp.exe that works with your version of Tecnai user interface program.

|_.Name:|_.File:|

| exposure adaptor|adaexp.exe|

h2. Packages required from NRAMM

h3. These are the sub-packages of myami that you will install with the python installer.

|_.Name:|_.Purpose:|

| leginon|modular TEM image acquisition|

| pyami|general functions|

| sinedon|Leginon/database interaction|

| pyscope|microscope control and monitoring|

| imageviewer|image viewing for tomography|

*For Gatan Camera or FEI Eagle Camera that uses TIA, comarray package needs to be install with python

Because numextension, comarray and libcv would require extra compilers if you build them yourself, we have created Windows installers for them for python 2.5 and made them available at http://emg.nysbc.org/projects/leginon/files.

h3. These are the Leginon v2.0 python 2.5 compiled packages installed through python installer on Windows.

|_.Downloadfile Name|_.Purpose:|

|numextension-2.0.0.win32-py2.5.exe|c extension for numerical processing|

|comarray-2.0.0.win32-py2.5.exe|com module output conversion to array|

|libCV-0.2.win32-py2.5.exe|small c library of algorithm from computer vision field|

h1. Installation

h2. 1. Register TecnaiCCD.dll, CAMC4.exe, and adaexep.exe

*  For example, from the command prompt:

<pre>adaexp.exe /regserver</pre>

h2. Install Python and supporting packages with their installers

Excute the installer files and follow the instructions.

h2. 2. Install the Windows Installer Files from Leginon website http://emg.nysbc.org/projects/leginon/files

Execute the installer files and follow the instructions.

h2. 3. Check out SVN Source Files from the repository

Use your mouse to do the following

* Create Leginon2.0 directory somewhere at your convenience

* Change directory into Leginon2.0

* Right-click the mouse botton in this directory window and select Tortoise svn

Checkout in the menu:

 !http://emg.nysbc.org/software/leginon/images/images/svnmenu.png!

* Set up svn checkout window like this to check out from http://emg.nysbc.org/svn/myami/branches/myami-2.0 to Leginon2.0

 !http://emg.nysbc.org/software/leginon/images/images/svnco.png!

h2. 4. Install the packages you downloaded from NRAMM svn repository

* Start a command line Window from Start Menu

* Install the package in each folder with commands such as

<pre>cd Your_Download_Place\Leginon2.0\leginon

c:\\python25\python.exe setup.py install</pre>

Then continue with the other packages, replacing leginon with the package name.

h2. 5. Configure leginon.cfg:

Follow the instructions in [[Configure leginon.cfg|Configure leginon.cfg]] located in the section for Linux installation but note the location of the configuration files follows. In addition, if the storage disk is mapped onto the Windows PC as drive Z, this mapping should be included in leginon.cfg. See above.

* Configurations for all users

 <pre> <Python directory>\Lib\site-packages\Leginon\config\leginon.cfg</pre>

 Example:

 <pre> C:\Python25\Lib\site-packages\Leginon\config\leginon.cfg</pre>

* Configurations for individual users

 <pre> <Home directory>\leginon.cfg</pre>

 Example:

 <pre> C:\Documents and Settings\Leginon User\leginon.cfg </pre>

* A skeleton (default) configuration file is available:

 <pre> C:\Python25\Lib\site-packages\Leginon\config\default.cfg</pre>

h2. 6. Configure sinedon.cfg:

Follow instruction in [[Configure sinedon.cfg]] in the section for Linux installation but note the location of the configuration files follows.

 * For all users, put sinedon.cfg with the installed package as

 <pre>C:\Python25\Lib\site-packages\sinedon\sinedon.cfg</pre>

 * Your home directory on Windows:

 <pre>C:\Documents and Settings\your_name></pre>

 * the skeleton sinedon configuration file is

 <pre>C:\Python25\Lib\site-packages\sinedon\examples\sinedon.cfg</pre>

h2. 7. Modify instruments.cfg:

* The file is in the installed pyscope directory

* Remove the Sim Tem and Sim Cam modules in the configuration.

* Add the modules for your microscope and camera. For example, if your microscope uses Tecnai Scripting Interface and you have a Gatan camera that you interface through DigitalMicrograph:

<pre>[tem]

class: tecnai.Tecnai

[camera]

class: gatan.Gatan</pre>

The file contains other examples of microscope and camera drivers that we distribute from NRAMM.

h2. 8. Run updatecom.py

From a command line window:

<pre>cd C:\python25\Lib\Site-Packages\pyScope

C:\python25\python.exe updatecom.py</pre>

The python window appears should say show the required type libraries it found:

<pre>

Generating .py files from type libraries...

initializing TEM Scripting Error, cannot find typelib for "TEM Scripting"

initializing Tecnai Scripting done.

initializing TOM Moniker done.

initializing Tecnai Low Dose Kit done.

initializing Tecnai Exposure Adaptor done.

initializing Tietz CCD Camera done.

</pre>

The output is of course depending on what is available on your microscope computer.  You should have either "Tecnai Scripting" or the pairing of  "TEM Scripting" and "TOM Moniker".

The script should generate a few files in C:\\python25\Lib\win32com\gen_py with seemly scrambled names such as BC0A2B03-19FF-11D3-AE00-00A024CBA50Cx0x1x9.py

h2. 9. Additional setup on Tietz cameras

Register the Tietz ping callback function.  From a command line window:

<pre>cd C:\python25\Lib\Site-Packages\pyScope

C:\python25\python.exe tietzping.py</pre>

h3. 10. For strange dimension cameras

Some camera dimensions are slightly larger than a standard size, for example, 2084 x 2084 instead of the standard 2048 x 2048.  Some software will have trouble dealing with these dimensions.  It is recommended to force the camera to the lower standard size (some multiple of 2^n).  Modify the function that gets camera dimension in tietz.py, gatan.py, or tia.py depending on which camera you are using.

*  Go to C:\Python25\Lib\site-packages\pyScope\

*  Edit tietz.py, gatan.py, or tia.py with a plain text editor

*  Find the function "getCameraSize" and replace its contents to force it to return a "hard coded" size.  For example:

<pre> def getCameraSize(self):

    return {'x': 2048, 'y': 2048}</pre>

h2. 10. Create Leginon and Leginon Client shortcut in Start menu menu under Leginon

This instruction applies to Windows XP.

 * Go to C:\Documents and Settings\All Users\Start Menu\Programs\ and create a new

folder named Leginon.

 * In another window, go to

 <pre>C:\Python25\Lib\site-packages\leginon</pre>

 * Create a shortcut from start-leginon.py as Leginon and a shortcut from launcher.py as Leginon Client.

 * Move the two shortcuts into

 <pre>C:\Documents and Settings\All Users\Start Menu\Programs\Leginon</pre>

h2. 11. Mapping Drives (Optional):

If you plan to run Leginon directly on the Windows machine, and your data files are served through a Samba server on a Linux machine, you will need to map the network drive. For example, if your Samba server has a hostname your_smbserver, and you have set up a share called [your_share_point] which points to /your_data_path/ and leginon data will be saved under a folder in /your_data_path/leginon/.

*  Start, My Computer

*  Tools menu, Map network drive

*  Use an unmapped drive such as Z:

 Enter shared path in Windows format as

 <pre>\\your_smbserver\your_share_point</pre>

*  Add the drive and the Linux path to leginon.cfg on the Windows machine as

 <pre>[Drive Mapping]

Z:/your_data_path</pre>

*  Add image path to leginon.cfg on the Windows machine in Linux format as

 <pre>[Images]

path:/your_data_path/leginon</pre>

h2.  Additional Software (Optional):

TightVNC (http://www.tightvnc.com) if you get tired of going into the microscope room just to open the column valves.

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[[Web Server Installation|< Web Server Installation]] | [[Steps involved in the installation| Steps needed for Installation Using Database Administration Tools >]]

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