Leginon

TEM¶

cs is the coefficient of spherical aberration in meters. Find out from TEM manufacture the proper value for your tem.
For example

[tem]
class: tecnai.Tecnai
cs: 2.0e-3

Camera¶

• Some Tietz camera may have an odd physical dimension. Use TCL/EMMenu program to find out the good dimension used in Tietz's own software and entered in this configuration.

For example

[camera]
class: gatan.Gatan
zplane: 50
height: 4096
width: 4096

zplane is a number that gives the order of camera. If a camera with low zplane value is going to acquire an image, Leginon will attempt to retract all cameras at higher zplane values.

• One computer for both tem and digital camera:
  

  [tem]
  class: tecnai.Tecnai
  cs: 2.0e-3
  [camera]
  class: gatan.Gatan
  zplane: 50
  

• Separate computers for the two instruments: configure only the instrument reside on the particular computer
  • On the computer that controls the tem:
    

    [tem]
    class: tecnai.Tecnai
    cs: 2.0e-3
    

  • On the computer that controls the digital camera:
    

    [camera]
    class: gatan.Gatan
    zplane: 50
    

• Two camera at the same plane:

  DE12 and DE Survey camera are retract/extend together, with the survey camera at off-axis. Therefore zplane should be set at the same value
  For example,
  

  [camera1]
  class: de.DE12
  zplane: 50
  
  [camera2]
  class: de.DESurvey
  zplane: 50
  

• Gatan Orius camera that is installed typically with Gatan K2/Falcon camera is technically on the same plane but is not capable of insertion unless K2 is retracted. On the other hand, K2 camera insertion includes a retraction of Orius at lower level of the function call in DM. Therefore, Orius is considered as a camera at lower zplane.
  

  [camera1]
  class: dmsem.GatanK2Base
  zplane: 50
  
  [camera2]
  class: dmsem.GatanOrius
  zplane: 49