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Krios1-K2-NYSBC-large shift » History » Revision 1
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Anchi Cheng, 03/21/2019 03:31 PM
Krios1-K2-NYSBC-large shift¶
Krios-K2 Presets used for 1.9 A mouse recombinant apoferritin reconstruction data collection at SEMC @ NYSBC¶
The same as Krios1-K2-NYSBC except
| Probe | Lens Series | Magnification: | Preset name: | Image Shift (x,y): | Dimension: | Binning: | Camera Dose (e/camera pixel/s): | Exposure Time (ms): | Specimen Dose Rate (e/A^2): | Spot Size: | Defocus (m): | C2 (um): | frame saving: | Camera |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| nano | SA | 1300** | hl | Aligned | 927x959 | 4 | 8 | 200 | 0.004 | 11 | -1.5e-4 | 70 | no | K2 |
| nano | SA | 22500 | en | 0,0 | 3710x3838 | 1 | 4.2 e/pixel/s and ~1.12 um on the specimen | 6000 | 58 | 6 | (-1e-6 to -2e-6) | 70 | 200 ms/frame |
**These two magnifications are add-on to standard SA lens series made for NYSBC Krios1. May not be available at other scopes.
- Note: no defocal pair was acquired in this experiment. Therefore, no ef preset.
Updated by Anchi Cheng over 5 years ago · 1 revisions