Leginon Homepage » History » Revision 9
Revision 8 (Neil Voss, 05/26/2010 11:34 AM) → Revision 9/46 (Neil Voss, 05/26/2010 11:39 AM)
h1. Leginon Main Page !>http://lh5.google.com/vossman77/RzJbEE3GhuI/AAAAAAAAASY/SSaOA7FZKwI/nramm.jpg?imgdl=1!:http://nramm.scripps.edu h2. Overview Leginon is a system designed for automated collection of images from a transmission electron microscope. h2. Contact information: Please email _amisoftware@scripps.edu_ with any questions. h2. Software Installation and Installation: bq. see [[Complete Installation|Complete Appion Installation]] h2. User Guide: bq. see the [[Leginon Manual|Leginon Manual]] [[Appion User Guide|Appion User Guide]] h2. Publications: h3. Primary Publication to Cite: * "Automated Molecular Microscopy: The New Leginon System" (2005), C Suloway, J Lander, G.C.; Stagg, S.M., Voss, N.R., Cheng, A., Fellmann, D., Pulokas, D Fellmann, A Cheng, F Guerra, J Quispe, S Stagg, CS Potter and B Carragher. J., Yoshioka, C., Irving, C., Mulder, A., Lau, P.W., _et al._ (2009). "Appion: an integrated, database-driven pipeline to facilitate EM image processing.". Journal of Structural Biology 151 (1): pp. 41-60. 166: 95-102. "PMID 19263523":http://www.ncbi.nlm.nih.gov/pubmed/19263523. h3. Additional References: * "Rapid, routine structure determination of macromolecular assemblies using electron microscopy: Current progress and remaining challenges", B. Carragher, D. Fellmann, F. Guerra, R.A. Milligan, F. Mouche, J. Pulokas, J. Quispe, C. Suloway, Y. Zhu and C.S. Potter, J. of Synchrotron radiation. 11, pp 83-85 (2004) * Voss, N.R.; Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, _et al._ (2010). "A relational database toolbox for cryoEM: Experience at one year and 50,000 images", D. Fellmann, J. Pulokas, R.A. Milligan, B. Carragher and C.S. Potter, J. ab initio 3-D reconstructions in single-particle electron microscopy.". Journal of Structural Biology, 137, p. 273-282 (2002) * "Leginon: An automated system for acquisition of images from vitreous ice specimens", B. Carragher, N. Kisseberth, D. Kriegman, R.A. Milligan, C.S. Potter, J. Pulokas and A. Reilein, Journal of Structual Biology 132, 33-45 (2000). 169 (3): 389-98. "PMID 20018246":http://www.ncbi.nlm.nih.gov/pubmed/20018246. * "Leginon: A system for fully automated acquisition of 1000 micrographs a day", C.S. Potter, H. Chu, B. Frey, C. Green, N. Kisseberth, T.J. Madden, K.L. Miller, K. Nahrstedt, J. Pulokas, A. Reilein, D. Tcheng, D. Weber and B. Carragher, Ultramicroscopy 77 (1999) 153-161. * "Automated cryoEM data acquisition and analysis of 284742 particles of GroEL." (2006) Stagg SM, Stagg, S.M.; Lander GC, Pulokas Quispe J, Fellmann D, Voss NR, Cheng A, Quispe JD, Mallick SP, Avila RM, Bradlow H, Bradlow S, Carragher B, Potter CS. J Struct Biol. 155 (3): pp. 470-81. PMID: 16762565. CS (2008). "A test-bed for optimizing high-resolution single particle reconstructions". Journal of Structural Biology 163 (1): 29-39. "PMID 18534866":http://www.ncbi.nlm.nih.gov/pubmed/18534866. h2. Software Availability and Licensing Information Appion is released under the "Apache License, Version 2.0":http://www.apache.org/licenses/LICENSE-2.0 View an [[myami code diagram|overview of the Appion code]] base found in the "myami" repository. h2. Youtube videos: * "An Introduction to Appion":http://www.youtube.com/watch?v=Wlxt_4yJKgA * "TiltPicker Demonstration":http://www.youtube.com/watch?v=z7BqGJczmjU