MSI-Ptolemy API information for developers » History » Version 3
Anchi Cheng, 03/15/2022 05:19 PM
| 1 | 1 | Anchi Cheng | h1. MSI-Ptolemy API information for developers |
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| 3 | h2. Square_Finding node: |
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| 5 | The square finding should operate on each gr image tile at its saved mrc dimension. |
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| 7 | # Shell script to call and specified in Blobs settings. |
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| 8 | 3 | Anchi Cheng | |
| 9 | The script should accept the same parameters and give json output as [[Setup_Ptolemy_CLI_shell_script]] |
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| 11 | 1 | Anchi Cheng | # Output json file is a list of square blob founds by the algorithm. For each item, it requires the following key, value pairs. |
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| 13 | h3. area: integer |
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| 15 | 2 | Anchi Cheng | > number of pixels the square opening on the gr tile image. |
| 16 | 1 | Anchi Cheng | |
| 17 | 2 | Anchi Cheng | h3. center: [float, float] |
| 18 | 1 | Anchi Cheng | |
| 19 | 2 | Anchi Cheng | > The coordinate of the square opening center [col, row] with [0,0] at the start of the data block in the mrc image. |
| 20 | 1 | Anchi Cheng | |
| 21 | 2 | Anchi Cheng | h3. score: float |
| 22 | 1 | Anchi Cheng | |
| 23 | 2 | Anchi Cheng | > The score of the blob as goodness of the blob as a good square. The higher the number the better. |
| 24 | 1 | Anchi Cheng | |
| 25 | 2 | Anchi Cheng | h3. brightness: float |
| 26 | 1 | Anchi Cheng | |
| 27 | 2 | Anchi Cheng | > Mean intensity value or equivalent in the square blob. This is saved as a part of statistics but not used for filtering. |
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| 29 | h3. vertices: [[float, float], [float, float], [float, float], .....] |
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| 31 | > convex-hull vertices used by leginon to determine if a cursor position is within the square blob. Same coordinate system as centers. |
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| 33 | h2. Example json: |
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| 35 | <pre> |
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| 36 | [{"vertices": [[600.4, 304.7], [502.5, 334.8], [532.7, 432.7], [630.5, 402.6]], "center": [566, 368], "area": 10488.9, "brightness": 194.2, "score": 0.3902}, |
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| 37 | {"vertices": [[464.1, 568.5], [370.0, 597.5], [399.2, 692.5], [493.3, 663.5]], "center": [431, 630], "area": 9785.5, "brightness": 193.7, "score": 0.2530}] |
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| 38 | </pre> |